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Mouse sperm generated in rats

Researchers report growing mouse stem cells into sperm in the body of rats

Date:
March 2, 2021
Source:
National Institutes of Natural Sciences
Summary:
Pluripotent stem cells (PSCs) can make all the tissues including gametes such as sperm and eggs. Researchers report a robust method for the generation of gametes from PSCs using the body of rats that cannot make gametes. Notably, they successfully generated mouse PSC-derived functional spermatids in rats. This approach will become a useful system for the generation of PSC-derived germ cells in the future.
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Making gametes such as sperm and eggs from pluripotent stem cells, primitive cells that can make all the tissues, greatly contributes to efficient reproduction of livestock animals and future assisted reproductive medicine. Researchers pave the way to achieve this goal using a body of xenogenic animals.

The researchers previously developed a method to grow stem cells into an entire organ in the body, so-called blastocyst complementation. The blastocyst is a structure of early embryos. If stem cells are transplanted into the blastocyst obtained from animals that cannot make a certain organ, the stem cells compensate the missing organ in the developing body, and make the entire organ. "We expected this method is also applicable to the efficient production of gametes," explains an author who led the study, Dr. Toshihiro Kobayashi.

A year ago, the researchers created a genetically modified rat that completely lacks sperm and eggs. They hypothesized that the rat can be used as an excellent host to grow exogenous stem cells into gametes.

The researchers firstly transplanted allogenic rat stem cells into blastocyst obtained from rats that are unable to make gametes, and confirmed all the gametes were derived from the stem cells. The generated gametes deliver the genetic information from the stem cells to the next generation, which enables efficient production of genetically modified rats.

Then, researchers tested whether xenogenic mouse stem cells can make mouse gametes in the body of rats. Remarkably, mouse germ cells including sperm and spermatids were observed in the testis of the rats, and the spermatids could fertilize with mouse eggs to produce healthy pups.

"Making gametes from stem cells even in the xenogenic environment is quite important for the application of this strategy," says another author led the study, Dr. Masumi Hirabayashi. "In the future, we may be able to use the method to preserve endangered species, since their stem cells are available due to iPS cell technology."


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Materials provided by National Institutes of Natural Sciences. Note: Content may be edited for style and length.


Journal Reference:

  1. Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M. Azim Surani, Masumi Hirabayashi. Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats. Nature Communications, 2021; 12 (1) DOI: 10.1038/s41467-021-21557-x

Cite This Page:

National Institutes of Natural Sciences. "Mouse sperm generated in rats." ScienceDaily. ScienceDaily, 2 March 2021. <www.sciencedaily.com/releases/2021/03/210302104752.htm>.
National Institutes of Natural Sciences. (2021, March 2). Mouse sperm generated in rats. ScienceDaily. Retrieved December 11, 2024 from www.sciencedaily.com/releases/2021/03/210302104752.htm
National Institutes of Natural Sciences. "Mouse sperm generated in rats." ScienceDaily. www.sciencedaily.com/releases/2021/03/210302104752.htm (accessed December 11, 2024).

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