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Boosting peptide design

Finding the holy grail of re-engineering peptide design -- for better antibiotics and beyond

May 10, 2021
Monash University
Peptides play a vital role with a huge range of medical uses including in antibiotics and anti-cancer drugs. Altering the structure of natural peptides to improve compounds is of great interest to scientists and industry. But how these peptides are produced still isn't clearly understood. Researchers have revealed a key aspect of peptide machineries in a new artcle that provides a key to the 'Holy Grail' of re-engineering peptides.

Peptides -- short strings of amino acids -- play a vital role in health and industry with a huge range of medical uses including in antibiotics, anti-inflammatory and anti-cancer drugs. They are also used in the cosmetics industry and for enhancing athletic performance.

Altering the structure of natural peptides to produce improved compounds is therefore of great interest to scientists and industry. But how the machineries that produce these peptides work still isn't clearly understood.

Monash Biomedicine Discovery Institute (BDI) scientist Associate Professor Max Cryle has revealed a key aspect of peptide machineries in a paper published in Nature Communications today.

The findings will allow Associate Professor Cryle to advance his lab's work into re-engineering glycopeptide antibiotics to counter the pressing global threat posed by antimicrobial resistance, and more broadly to improving the properties of peptides generally.

"Peptide synthesis machineries are often largely modular assembly lines, with each module comprised of different component parts. Changing what you make in these assembly lines, that is, peptides with new bioactivities, is a 'holy grail' in re-design," Associate Professor Cryle said.

"One of the things we tried to understand in this study was where the selectivity of these machineries come from -- they're very selective for making one specific peptide and understanding where this specificity comes from is a bit of a mystery," he said.

"We were able to structurally characterise a part of such a machinery that generates the links within the peptides at a stage that hasn't been previously determined. What we showed is that these domains responsible for the linking of amino acids into peptides don't play a general role in selecting the amino acids during this process.

"This is good news from a re-engineering point of view because it means we don't need to concern ourselves with changing multiple pieces of the machinery to make single amino acid changes, we just need to focus on changing the building block that goes in -- and that's quite promising."

Associate Professor Cryle led a multidisciplinary team of scientists who enlisted a variety of techniques to model the peptide structures including using the Australian Synchrotron for X-ray crystallography along with chemical and biochemical techniques. He collaborated with groups in Canberra, Brisbane and Germany who helped with computational modelling and bioinformatics.

"Our ability to understand the enzymes that make natural peptides is key to our ability to produce improved ones to target issues like antimicrobial resistance," he said. "Now we can actually start to think about ways to change the machinery's acceptance of different building blocks and in this way we can make new peptides with improved antibacterial properties," he said.

In the future, a collaboration with Dr Evi Stegmann's group at the University of Tübingen in Germany will help translate the findings from a theoretical lab solution to eventually developing a commercial-scale production of new and improved antibiotics, he said.

Dr Thierry Izore, who has since left the Monash BDI to work at Medpace, and PhD student Candace Ho from the University of Warwick, UK, are equal first authors.

The work is linked to the University of Warwick, UK. Associate Professor Cryle is a member of the ARC Centre of Excellence for Innovations in Peptide and Protein Science.

Story Source:

Materials provided by Monash University. Note: Content may be edited for style and length.

Journal Reference:

  1. Thierry Izoré, Y. T. Candace Ho, Joe A. Kaczmarski, Athina Gavriilidou, Ka Ho Chow, David L. Steer, Robert J. A. Goode, Ralf B. Schittenhelm, Julien Tailhades, Manuela Tosin, Gregory L. Challis, Elizabeth H. Krenske, Nadine Ziemert, Colin J. Jackson, Max J. Cryle. Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity. Nature Communications, 2021; 12 (1) DOI: 10.1038/s41467-021-22623-0

Cite This Page:

Monash University. "Boosting peptide design." ScienceDaily. ScienceDaily, 10 May 2021. <>.
Monash University. (2021, May 10). Boosting peptide design. ScienceDaily. Retrieved November 27, 2023 from
Monash University. "Boosting peptide design." ScienceDaily. (accessed November 27, 2023).

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