Featured Research

from universities, journals, and other organizations

Widely Used Virus Assay Shown Unreliable When Compared To Other Methods

Date:
October 23, 2009
Source:
Virginia Tech
Summary:
In the course of doing research on the mosquito-borne pathogens chikungunya virus and o' nyong-nyong virus, researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

The research appears in the Wednesday, October 14, 2009, issue of PLoS ONE, in the article, "Accurate Strand-Specific Quantification of Viral RNA," by Nicole E. Plaskon of Richmond Va., a Master of Science in life sciences candidate in the College of Agriculture and Life Sciences, and entomology Assistant Professors Zach N. Adelman and Kevin M. Myles, all with the Fralin Life Science Institute..

CHIKV has sickened millions of people in India and Africa in the last five years -- 1.3 million in India alone. ONNV has also previously caused large outbreaks of human disease with cases numbering in the millions. In studying virus infection of the mosquito, the Virginia Tech researchers developed a novel assay that detects and measures anti-genomic copies of the viral genome. This differs from traditional assays that simply measure viral nucleic acids associated with infection, regardless of origin.

"The application of real-time PCR to the detection and quantification of specific strands of viral RNA is becoming an increasingly important tool in the study of RNA viruses. As a result, multiple types of ssqPCR assays have been described and are in widespread use. However, no study has yet determined if the accuracy with which the different types of assays detect and quantify specific strands of viral RNA are equivalent. It turns out they are not, and the most frequently used method is the most error prone," said Myles.

"A less frequently used ssqPCR assay turned out to be more accurate," said Adelman.

"The fact that many labs have been using assays prone to error may have led to some wrong conclusions," Adelman said. "Using the more accurate assays will lead to more accurate conclusions and better science."

Although Myles and Adelman developed their assays for CHIK and ONNV, the results should help improve the design of ssqPCR assays for the study of other RNA viruses as well.


Story Source:

The above story is based on materials provided by Virginia Tech. Note: Materials may be edited for content and length.


Journal Reference:

  1. Nicole E. Plaskon, Zach N. Adelman, Kevin M. Myles. Accurate Strand-Specific Quantification of Viral RNA. PLoS ONE, 2009; 4 (10): e7468 DOI: 10.1371/journal.pone.0007468

Cite This Page:

Virginia Tech. "Widely Used Virus Assay Shown Unreliable When Compared To Other Methods." ScienceDaily. ScienceDaily, 23 October 2009. <www.sciencedaily.com/releases/2009/10/091021212245.htm>.
Virginia Tech. (2009, October 23). Widely Used Virus Assay Shown Unreliable When Compared To Other Methods. ScienceDaily. Retrieved September 23, 2014 from www.sciencedaily.com/releases/2009/10/091021212245.htm
Virginia Tech. "Widely Used Virus Assay Shown Unreliable When Compared To Other Methods." ScienceDaily. www.sciencedaily.com/releases/2009/10/091021212245.htm (accessed September 23, 2014).

Share This



More Plants & Animals News

Tuesday, September 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Ice Age Wooly Mammoth Remains for Sale

Raw: Ice Age Wooly Mammoth Remains for Sale

AP (Sep. 23, 2014) A rare, well-preserved skeleton of a woolly mammoth is going on sale at Summers Place Auctions hope the 11.5-foot tall, almost intact specimen will fetch between $245,000 to $409,000. (Sept. 23) Video provided by AP
Powered by NewsLook.com
Fox Bites Conn. Student And School Staffers In Rare Attack

Fox Bites Conn. Student And School Staffers In Rare Attack

Newsy (Sep. 23, 2014) A fox attacked a second-grade boy at a Connecticut elementary school Monday. It also attacked two school staff members and a woman and her dog. Video provided by Newsy
Powered by NewsLook.com
Will Living Glue Be A Thing?

Will Living Glue Be A Thing?

Newsy (Sep. 23, 2014) Using proteins derived from mussels, engineers at MIT have made a supersticky underwater adhesive. They're now looking to make "living glue." Video provided by Newsy
Powered by NewsLook.com
Raw: Tiger Kills Man at India Zoo

Raw: Tiger Kills Man at India Zoo

AP (Sep. 23, 2014) A white tiger killed a young man who climbed over a fence at the New Delhi zoo and jumped into the animal's enclosure on Tuesday, a spokesman said. (Sept. 23) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins