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New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues

Date:
May 11, 2011
Source:
Ruhr-Universitaet-Bochum
Summary:
Researchers in Germany have produced an antibody that allows them to distinguish the numerous types of stem cells in the nervous system better than before. The antibody 5750 recognizes a specific sugar residue on the cell surface, which is called LewisX. The research group has now been able to use LewisX for the first time to separate different types of stem cells.

Two types of stem cells were stained with the new Bochum antibody 5750 (red) and the conventional antibody 487 (green). They can be clearly separated, since the antibodies recognise different LewisX sugar residues.
Credit: American Society for Biochemistry and Molecular Biology

Researchers in Bochum have produced an antibody that allows them to distinguish the numerous types of stem cells in the nervous system better than before.

"In order to use stem cells for therapeutic purposes, it is important to be able to distinguish between the different types," explained Eva Hennen of the RUB Department of Cell Morphology and Molecular Neurobiology (Faculty of Biology and Biotechnology). The antibody 5750 recognises a specific sugar residue on the cell surface, which is called LewisX. The research group led by Prof. Dr. Andreas Faissner has now been able to use LewisX for the first time to separate different types of stem cells. The researchers report on their results in the Journal of Biological Chemistry.

Unexpected sugar diversity

Antibodies that recognise the LewisX sugar residue are used routinely to identify so-called neural stem cells from which the various cells of the nervous system originate. Prof. Faissner's team has now shown that the designation "LewisX" does not just cover a single sugar motif, but a whole range of different sugar residues. Different types of neural stem cells are equipped with individual combinations of LewisX sugar residues on their cell surface. The new Bochum antibody 5750 recognises a different LewisX sugar residue to the antibodies previously used. "This sugar diversity could also be interesting for cancer diagnosis" Prof. Faissner explained, "because LewisX sugars have also been detected on tumour cells."

Identifying properties of stem cells

With the aid of the new antibody 5750, certain types of neural stem cells can be isolated from a mixture of different cell types. The aim of Prof. Faissner's research group is now to examine the properties of the stem cells which carry the LewisX sugar residues. The researchers have already found out that the LewisX motif on the cell surface changes when the stem cells develop further -- for example into oligodendrocytes, which form the insulation layer of the nerve cells, or into nerve cells themselves.


Story Source:

The above story is based on materials provided by Ruhr-Universitaet-Bochum. Note: Materials may be edited for content and length.


Journal Reference:

  1. E. Hennen, T. Czopka, A. Faissner. Structurally Distinct LewisX Glycans Distinguish Subpopulations of Neural Stem/Progenitor Cells. Journal of Biological Chemistry, 2011; 286 (18): 16321 DOI: 10.1074/jbc.M110.201095

Cite This Page:

Ruhr-Universitaet-Bochum. "New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues." ScienceDaily. ScienceDaily, 11 May 2011. <www.sciencedaily.com/releases/2011/05/110510074436.htm>.
Ruhr-Universitaet-Bochum. (2011, May 11). New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues. ScienceDaily. Retrieved August 2, 2014 from www.sciencedaily.com/releases/2011/05/110510074436.htm
Ruhr-Universitaet-Bochum. "New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues." ScienceDaily. www.sciencedaily.com/releases/2011/05/110510074436.htm (accessed August 2, 2014).

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