Featured Research

from universities, journals, and other organizations

New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues

Date:
May 11, 2011
Source:
Ruhr-Universitaet-Bochum
Summary:
Researchers in Germany have produced an antibody that allows them to distinguish the numerous types of stem cells in the nervous system better than before. The antibody 5750 recognizes a specific sugar residue on the cell surface, which is called LewisX. The research group has now been able to use LewisX for the first time to separate different types of stem cells.

Two types of stem cells were stained with the new Bochum antibody 5750 (red) and the conventional antibody 487 (green). They can be clearly separated, since the antibodies recognise different LewisX sugar residues.
Credit: American Society for Biochemistry and Molecular Biology

Researchers in Bochum have produced an antibody that allows them to distinguish the numerous types of stem cells in the nervous system better than before.

"In order to use stem cells for therapeutic purposes, it is important to be able to distinguish between the different types," explained Eva Hennen of the RUB Department of Cell Morphology and Molecular Neurobiology (Faculty of Biology and Biotechnology). The antibody 5750 recognises a specific sugar residue on the cell surface, which is called LewisX. The research group led by Prof. Dr. Andreas Faissner has now been able to use LewisX for the first time to separate different types of stem cells. The researchers report on their results in the Journal of Biological Chemistry.

Unexpected sugar diversity

Antibodies that recognise the LewisX sugar residue are used routinely to identify so-called neural stem cells from which the various cells of the nervous system originate. Prof. Faissner's team has now shown that the designation "LewisX" does not just cover a single sugar motif, but a whole range of different sugar residues. Different types of neural stem cells are equipped with individual combinations of LewisX sugar residues on their cell surface. The new Bochum antibody 5750 recognises a different LewisX sugar residue to the antibodies previously used. "This sugar diversity could also be interesting for cancer diagnosis" Prof. Faissner explained, "because LewisX sugars have also been detected on tumour cells."

Identifying properties of stem cells

With the aid of the new antibody 5750, certain types of neural stem cells can be isolated from a mixture of different cell types. The aim of Prof. Faissner's research group is now to examine the properties of the stem cells which carry the LewisX sugar residues. The researchers have already found out that the LewisX motif on the cell surface changes when the stem cells develop further -- for example into oligodendrocytes, which form the insulation layer of the nerve cells, or into nerve cells themselves.


Story Source:

The above story is based on materials provided by Ruhr-Universitaet-Bochum. Note: Materials may be edited for content and length.


Journal Reference:

  1. E. Hennen, T. Czopka, A. Faissner. Structurally Distinct LewisX Glycans Distinguish Subpopulations of Neural Stem/Progenitor Cells. Journal of Biological Chemistry, 2011; 286 (18): 16321 DOI: 10.1074/jbc.M110.201095

Cite This Page:

Ruhr-Universitaet-Bochum. "New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues." ScienceDaily. ScienceDaily, 11 May 2011. <www.sciencedaily.com/releases/2011/05/110510074436.htm>.
Ruhr-Universitaet-Bochum. (2011, May 11). New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues. ScienceDaily. Retrieved April 16, 2014 from www.sciencedaily.com/releases/2011/05/110510074436.htm
Ruhr-Universitaet-Bochum. "New antibody for cell labeling: Stem cells can be distinguished on the basis of sugar residues." ScienceDaily. www.sciencedaily.com/releases/2011/05/110510074436.htm (accessed April 16, 2014).

Share This



More Health & Medicine News

Wednesday, April 16, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Ebola Outbreak Now Linked To 121 Deaths

Ebola Outbreak Now Linked To 121 Deaths

Newsy (Apr. 15, 2014) The ebola virus outbreak in West Africa is now linked to 121 deaths. Health officials fear the virus will continue to spread in urban areas. Video provided by Newsy
Powered by NewsLook.com
Cognitive Function: Is It All Downhill From Age 24?

Cognitive Function: Is It All Downhill From Age 24?

Newsy (Apr. 15, 2014) A new study out of Canada says cognitive motor performance begins deteriorating around age 24. Video provided by Newsy
Powered by NewsLook.com
How Mt. Everest Helped Scientists Research Diabetes

How Mt. Everest Helped Scientists Research Diabetes

Newsy (Apr. 15, 2014) British researchers were able to use Mount Everest's low altitudes to study insulin resistance. They hope to find ways to treat diabetes. Video provided by Newsy
Powered by NewsLook.com
Carpenter's Injury Leads To Hundreds Of 3-D-Printed Hands

Carpenter's Injury Leads To Hundreds Of 3-D-Printed Hands

Newsy (Apr. 14, 2014) Richard van As lost all fingers on his right hand in a woodworking accident. Now, he's used the incident to create a prosthetic to help hundreds. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins