Featured Research

from universities, journals, and other organizations

The cell that isn't: New technique captures division of membrane-less cells

Date:
January 18, 2013
Source:
European Molecular Biology Laboratory (EMBL)
Summary:
A new technique allows scientists to study cell division without a cell membrane. There are several advantages: it can be physically constrained and manipulated; one can access nuclei which is normally buried deep in an opaque embryo; the method ican be combined with a wide-range of fruit fly genetics techniques. The method has revealed that, surprisingly, confined space not enough to restrict spindle size.

A new technique captures the division of membrane-less cells (red: chromosomes; green: mitotic spindle).
Credit: EMBL

A new technique allows scientists to study cell division without a cell membrane. There are several advantages: it can be physically constrained and manipulated; one can access nuclei which is normally buried deep in an opaque embryo; the method ican be combined with a wide-range of fruit fly genetics techniques. The method has revealed that, surprisingly, confined space not enough to restrict spindle size.

Related Articles


This may look like yet another video (http://www.youtube.com/watch?v=7krCUBuHvpw&feature=youtu.be) of a dividing cell, but there's a catch. You are looking at chromosomes (red) being pulled apart by the mitotic spindle (green), but it's not a cell, because there's no cell membrane. Like a child sucking an egg out of its shell, Ivo Telley from the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, removed these cellular 'innards' from a fruit fly embryo, at a stage when it is essentially a sac full of membrane-less 'cells' that divide and divide without building physical barriers to separate themselves from each other.

"It's the first time we can study ongoing cell division without the cell membrane, and that means we can physically manipulate things," says Telley, "so we can uncover the physical forces involved, and see what are the constraints."

The new technique is described in detail today in Nature Protocols, and has already led Telley and colleagues to a surprising discovery. They found that, although successive divisions fill the embryo with more and more material, leaving less and less space for each spindle, and spindles become smaller as the embryo develops, simply squeezing the 'cell' into tighter quarters doesn't make it produce a smaller spindle.

Combined with the genetic manipulation approaches commonly used in fruit fly studies, the scientists believe their new technique will help to unravel this and other mysteries of how a cell becomes two.

The work, which started in Thomas Surrey's lab at EMBL, was carried out by Telley and Imre Gαspαr in Anne Ephrussi's lab at EMBL. Surrey is now at Cancer Research UK.


Story Source:

The above story is based on materials provided by European Molecular Biology Laboratory (EMBL). Note: Materials may be edited for content and length.


Journal Reference:

  1. Ivo A Telley, Imre Gαspαr, Anne Ephrussi, Thomas Surrey. A single Drosophila embryo extract for the study of mitosis ex vivo. Nature Protocols, 2013; 8 (2): 310 DOI: 10.1038/nprot.2013.003

Cite This Page:

European Molecular Biology Laboratory (EMBL). "The cell that isn't: New technique captures division of membrane-less cells." ScienceDaily. ScienceDaily, 18 January 2013. <www.sciencedaily.com/releases/2013/01/130118064725.htm>.
European Molecular Biology Laboratory (EMBL). (2013, January 18). The cell that isn't: New technique captures division of membrane-less cells. ScienceDaily. Retrieved March 6, 2015 from www.sciencedaily.com/releases/2013/01/130118064725.htm
European Molecular Biology Laboratory (EMBL). "The cell that isn't: New technique captures division of membrane-less cells." ScienceDaily. www.sciencedaily.com/releases/2013/01/130118064725.htm (accessed March 6, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, March 6, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Giant Panda Goes Walkabout in Southwest China

Giant Panda Goes Walkabout in Southwest China

AFP (Mar. 6, 2015) — A giant panda goes walkabout alone at night in southwest China. Duration: 00:37 Video provided by AFP
Powered by NewsLook.com
Nesting Bald Eagle Covered in Snow Up to Its Neck

Nesting Bald Eagle Covered in Snow Up to Its Neck

Buzz60 (Mar. 6, 2015) — The Pennsylvania State Game Commission captured amazing shots of a nesting bald eagle who stayed on its nest during a snowstorm, even when the snow piled all the way up to its neck. Jen Markham (@jenmarkham) has the story. Video provided by Buzz60
Powered by NewsLook.com
Lack of Snow Pushes Alaska Sled Dog Race North

Lack of Snow Pushes Alaska Sled Dog Race North

AP (Mar. 6, 2015) — A shortage of snow has forced Alaska&apos;s Iditarod Trail Sled Dog Race to move 300 miles north to Fairbanks. The ceremonial start through downtown Anchorage will take place this weekend, using snow stockpiled earlier this winter. (March 6) Video provided by AP
Powered by NewsLook.com
Praying Mantis Looks Long Before It Leaps

Praying Mantis Looks Long Before It Leaps

Reuters - Innovations Video Online (Mar. 5, 2015) — Slowed-down footage of the leaps of praying mantises show the insect&apos;s extraordinary precision, say researchers. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins