Transport proteins are responsible for moving materials such as nutrients and metabolic products through a cell's outer membrane, which seals and protects all living cells, to the cell's interior. These transported molecules include sugars, which can be used to fuel growth or to respond to chemical signals of activity or stress outside of the cell. Measuring the activity of transporter proteins in a living organism has been a challenge for scientists, because the methods are difficult, often require the use of radioactive tracers, and are hard to use in intact tissues and organs.
A team led by Wolf Frommer, director of Carnegie's Plant Biology Department, has now developed a groundbreaking new way to overcome this technology gap. This new technology has major implications not just for plant biology, but also for cellular biology research in every type of organism, including humans. Their work is published by eLife.
"With the advent of biosensors, we could measure energy dynamics and concentrations of various cellular intermediates, which allowed us to get a first-level picture of metabolic networks," Frommer said. "But we had not been able to directly follow enzyme or transporter activity or to monitor their regulation in a live organism."
Frommer and his team hypothesized that it may be possible to probe transport activity by spying on the structural rearrangements that a transporter undergoes as it moves its target molecule across the membrane barrier. They decided to do this by encoding environmentally sensitive fluorescent tags in the cell's DNA.
The team -- which included Carnegie's Roberto De Michele, Cindy Ast, Chen-Hsun Ho, Viviane Lanquar, and Guido Grossman -- focused on the transporter responsible for moving the ammonium into a cell. This activity is very important in plants, fungi, and bacteria, because ammonium serves as the key source of nitrogen in these organisms. But in excess ammonium becomes toxic. Therefore, its concentration must be very carefully regulated. The transporter for ammonium is conserved in plants, fungi, and, bacteria. It is also present in humans, where it is generally known as the Rhesus factor and plays an important role in kidney function and male fertility.
The team's approach has provided new insights into how the plant ammonium transporter works. And their sensor concept is expected to find many other applications to monitor other types of transporters and transporters in other organisms outside of the plant kingdom and even enzymes.
"For example, in humans such sensors could be used to help understand neurotransmitter transport in the brain or identify new drugs targets," Frommer said.
- Roberto De Michele, Cindy Ast, Dominique Loquι, Cheng-Hsun Ho, Susana LA Andrade, Viviane Lanquar, Guido Grossmann, Sφren Gehne, Michael U Kumke, Wolf B. Fluorescent sensors reporting the activity of ammonium transceptors in live cells. eLife, 2013 DOI: 10.7554/eLife.00800
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