Featured Research

from universities, journals, and other organizations

Inhibition of oral biofilm, cell-cell communication using natural-products derivatives

Date:
March 20, 2014
Source:
International & American Associations for Dental Research
Summary:
Many plant metabolites and structurally similar derivatives have been identified as inhibitors of bacterial biofilm formation and quorum sensing (QS). Previously, the researchers of this study demonstrated biofilm and QS inhibition using modified cysteines, similar to those produced by the tropical plant Petiveria alliacea. In this study the researchers expanded their compound library to examine structure-activity relationships for biofilm and QS inhibition.

During the 43rd Annual Meeting & Exhibition of the American Association for Dental Research, held in conjunction with the 38th Annual Meeting of the Canadian Association for Dental Research, Steve Kasper, SUNY College of Nanoscale Science and Engineering, Albany, will present research titled "Inhibition of Oral Biofilm and Cell-cell Communication Using Natural-products Derivatives."

Many plant metabolites and structurally similar derivatives have been identified as inhibitors of bacterial biofilm formation and quorum sensing (QS). Previously, the researchers of this study demonstrated biofilm and QS inhibition using modified cysteines, similar to those produced by the tropical plant Petiveria alliacea. In this study the researchers expanded their compound library to examine structure-activity relationships for biofilm and QS inhibition.

Using a microplate-based screening approach, they observed the biofilm formation by three indigenous oral Gram-positive bacteria: Streptococcus mutans UA159, Streptococcus sanguis 10556, and Actinomyces oris MG1. Bacteria were grown in the presence of inhibitory compounds and analyzed using fluorescent staining for biomass and via confocal microscopy.

Compounds were also tested in a Vibrio harveyi QS reporter which responds to autoinducer-2 (AI-2) signaling (interspecies) but not acyl-homoserine lactone signaling (intraspecies). Reverse transcriptase real-time PCR and global RNA sequencing (RNAseq) were used to study modified genetic expression in S. mutans UA159 in the presence of select compounds from our library.

From their 46 compound library, six were capable of inhibiting biofilm formation in all three species tested at a concentration of 1mM. All six compounds are structurally similar to S-ribosyl homocysteine, the precursor for autoinducer-2 biosynthesis. These compounds also reduced bioluminescence in V. harveyi BB170, indicating inhibition of AI-2 based QS. Gene expression analysis showed distinct down-regulation of genes previously related to quorum sensing and/or biofilm formation in S. mutans.

The use of plant-inspired cysteine derivatives to inhibit bacterial virulence may serve as a novel tool to improve oral health. The researchers of this study propose that the compounds used in this study may inhibit biofilm formation by interrupting bacterial communication pathways, particularly in AI-2 biosynthetic reactions. Since their library is derived from eukaryotic (plant) origins, this study may provide initial evidence of interkingdom signaling, which has implications for studies of the human microbiome.


Story Source:

The above story is based on materials provided by International & American Associations for Dental Research. Note: Materials may be edited for content and length.


Cite This Page:

International & American Associations for Dental Research. "Inhibition of oral biofilm, cell-cell communication using natural-products derivatives." ScienceDaily. ScienceDaily, 20 March 2014. <www.sciencedaily.com/releases/2014/03/140320101507.htm>.
International & American Associations for Dental Research. (2014, March 20). Inhibition of oral biofilm, cell-cell communication using natural-products derivatives. ScienceDaily. Retrieved August 21, 2014 from www.sciencedaily.com/releases/2014/03/140320101507.htm
International & American Associations for Dental Research. "Inhibition of oral biofilm, cell-cell communication using natural-products derivatives." ScienceDaily. www.sciencedaily.com/releases/2014/03/140320101507.htm (accessed August 21, 2014).

Share This




More Health & Medicine News

Thursday, August 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Possible Ebola Patient in Isolation at California Hospital

Possible Ebola Patient in Isolation at California Hospital

Reuters - US Online Video (Aug. 20, 2014) — A patient who may have been exposed to the Ebola virus is in isolation at the Kaiser Permanente South Sacramento Medical Center. Linda So reports. Video provided by Reuters
Powered by NewsLook.com
Raw: World's Oldest Man Lives in Japan

Raw: World's Oldest Man Lives in Japan

AP (Aug. 20, 2014) — A 111-year-old Japanese was certified as the world's oldest man by Guinness World Records on Wednesday. Sakari Momoi, a native of Fukushima in northern Japan, was given a certificate at a hospital in Tokyo. (Aug. 20) Video provided by AP
Powered by NewsLook.com
Do More Wedding Guests Make A Happier Marriage?

Do More Wedding Guests Make A Happier Marriage?

Newsy (Aug. 20, 2014) — A new study found couples who had at least 150 guests at their weddings were more likely to report being happy in their marriages. Video provided by Newsy
Powered by NewsLook.com
Ebola-Hit Sierra Leone's Freetown a City on Edge

Ebola-Hit Sierra Leone's Freetown a City on Edge

AFP (Aug. 19, 2014) — Residents of Sierra Leone's capital voice their fears as the Ebola virus sweeps through west Africa. Duration: 00:56 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins