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Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics, reveals evidence of recent reticulate evolution

Date:
March 7, 2016
Source:
Ghent University
Summary:
The polyphagous parthenogenetic root-knot nematodes of the genus Meloidogyne are considered to be the most significant nematode pest in sub-tropical and tropical agriculture. Despite the crucial need for correct diagnosis, identification of these pathogens remains problematic. Scientists have refined the identification of the nematodes.
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The polyphagous parthenogenetic root-knot nematodes of the genus Meloidogyne are considered to be the most significant nematode pest in sub-tropical and tropical agriculture. Despite the crucial need for correct diagnosis, identification of these pathogens remains problematic. Scientists of Ghent University (Belgium), the National Plant Protection Organization (the Netherlands) en het International Institute of Tropical Agriculture (Nigeria) refined the identification of the nematodes.

The traditionally used diagnostic strategies, including morphometrics, host-range tests, biochemical and molecular techniques, now appear to be unreliable due to the recently-suggested hybrid origin of root-knot nematodes. In order to determine a suitable barcode region for these pathogens nine quickly-evolving mitochondrial coding genes were screened. Resulting haplotype networks revealed closely related lineages indicating a recent speciation, an anthropogenic-aided distribution through agricultural practices, and evidence for reticulate evolution within M. arenaria. Nonetheless, nucleotide polymorphisms harbor enough variation to distinguish these closely-related lineages.

Furthermore, completeness of lineage sorting was verified by screening 80 populations from widespread geographical origins and variable hosts. Importantly, our results indicate that mitochondrial haplotypes are strongly linked and consistent with traditional esterase isozyme patterns, suggesting that different parthenogenetic lineages can be reliably identified using mitochondrial haplotypes. The study indicates that the barcode region Nad5 can reliably identify the major lineages of tropical root-knot nematodes.


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Materials provided by Ghent University. Note: Content may be edited for style and length.


Journal Reference:

  1. Toon Janssen, Gerrit Karssen, Myrtle Verhaeven, Danny Coyne, Wim Bert. Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports, 2016; 6: 22591 DOI: 10.1038/srep22591

Cite This Page:

Ghent University. "Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics, reveals evidence of recent reticulate evolution." ScienceDaily. ScienceDaily, 7 March 2016. <www.sciencedaily.com/releases/2016/03/160307093508.htm>.
Ghent University. (2016, March 7). Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics, reveals evidence of recent reticulate evolution. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2016/03/160307093508.htm
Ghent University. "Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics, reveals evidence of recent reticulate evolution." ScienceDaily. www.sciencedaily.com/releases/2016/03/160307093508.htm (accessed May 23, 2017).

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