Science News
from research organizations

Totally new kind of 'mark' discovered in human cell nucleus

Will lead to the clarification of mechanism of development of metabolism and cardiovascular disorder

Date:
July 1, 2016
Source:
Osaka University
Summary:
Scientists have verified the presence of a protein modification that is a unique mark in human cell nucleus. This protein modification, Histone H4 lysine 20 acetylation (H4K20ac), was only discovered in plant cells and its existence in mammalian cells has been indirectly proven. Their study suggests that H4K20ac is associated with gene repression. This new discovery of H4K20ac may lead to further clarification of the mechanisms in disease progression.
Share:
FULL STORY

It was accepted that histone acetylation modifications are implicated only in gene activation "switch on".
Credit: Osaka University

A Japanese research group verified the presence of a protein modification that is a unique mark in human cell nucleus. This protein modification, Histone H4 lysine 20 acetylation (H4K20ac), was only discovered in plant cells and its existence in mammalian cells has been indirectly proven. Their study suggests that H4K20ac is associated with gene repression. This new discovery of H4K20ac may lead to further clarification of the mechanisms in disease progression.

In recent years, it has been known that epigenetic factors, factors outside of the genetic sequence, are deeply involved in the development of various diseases. Histone, a component of chromatin consisting of DNA, receives post-translational modification for changing the function of protein as an epigenetic factor. It was accepted that histone acetylation modifications are implicated only in gene activation,"switch on."

A research group was led by Yoshitaka Isaka, professor, and Shiro Takahara, endowed chair professor, and Junya Kaimori, endowed chair professor at the Graduate School of Medicine together with Toshifumi Takao, professor at the Institute for Protein Research, Osaka University, Hiroshi Kimura, professor at the Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, and Professor Yasuyuki Okawa, professor at Medical Institute of Bioregulation, Kyushu University.

This group made antibodies against H4K20ac. Although known acetyliation was enriched around promoters of highly expressed genes, this group, by using a chromatin immunoprecipitation method, next-generation sequencing, and supercomputers, found that H4K20ac, a new type of histone modification, was enriched around promoters of minimally expressed genes.

It is expected that the mechanism of development of various diseases such as metabolic disorders including diabetes, cardiovascular diseases including cardiomegaly, kidney disorders, and cancer will be elucidated by using H4K20ac, a new type of histone modification discovered by this group.


Story Source:

Materials provided by Osaka University. Note: Content may be edited for style and length.


Journal Reference:

  1. Jun-Ya Kaimori, Kazumitsu Maehara, Yoko Hayashi-Takanaka, Akihito Harada, Masafumi Fukuda, Satoko Yamamoto, Naotsugu Ichimaru, Takashi Umehara, Shigeyuki Yokoyama, Ryo Matsuda, Tsuyoshi Ikura, Koji Nagao, Chikashi Obuse, Naohito Nozaki, Shiro Takahara, Toshifumi Takao, Yasuyuki Ohkawa, Hiroshi Kimura, Yoshitaka Isaka. Histone H4 lysine 20 acetylation is associated with gene repression in human cells. Scientific Reports, 2016; 6: 24318 DOI: 10.1038/srep24318

Cite This Page:

Osaka University. "Totally new kind of 'mark' discovered in human cell nucleus: Will lead to the clarification of mechanism of development of metabolism and cardiovascular disorder." ScienceDaily. ScienceDaily, 1 July 2016. <www.sciencedaily.com/releases/2016/07/160701120354.htm>.
Osaka University. (2016, July 1). Totally new kind of 'mark' discovered in human cell nucleus: Will lead to the clarification of mechanism of development of metabolism and cardiovascular disorder. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2016/07/160701120354.htm
Osaka University. "Totally new kind of 'mark' discovered in human cell nucleus: Will lead to the clarification of mechanism of development of metabolism and cardiovascular disorder." ScienceDaily. www.sciencedaily.com/releases/2016/07/160701120354.htm (accessed May 23, 2017).

RELATED STORIES