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New microscope uses adaptable mirror to create clearer images

Date:
June 30, 2017
Source:
National Institute of Biomedical Imaging and Bioengineering
Summary:
A new microscope merges different microscopy methods to increase resolution and contrast in thick biological samples. A key component of the method is two-photon microscopy, used to generate a small point of light deep inside the sample. By moving this light throughout the sample and collecting information on how it is being distorted, researchers are able to adjust the shape of the mirror to cancel out the distortions, thus creating a clear image of the whole sample.
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A new microscope merges different microscopy methods to increase resolution and contrast in thick biological samples. Developed by Hari Shroff, Ph.D., and his team at the National Institute of Biomedical Imaging and Bioengineering (NIBIB), this new microscope improves on a previously developed microscope, combining two-photon laser scanning microscopy (2PM) and instant structured illumination microscopy (ISIM) by including adaptive optics (AO) to rapidly correct distortions.

Shroff and his team tackled a major problem that researchers encounter when attempting to image thick tissue samples. It can be difficult to get a clear image within a thick sample. This is not unlike looking down into a pool and seeing a ball at the bottom. The image, seen through the water, doesn't look very crisp. Astronomers have the same problem when attempting to look at distant objects through the earth's atmosphere. Shroff incorporated a technique called adaptive optics to his latest super-resolution microscope to help solve this problem of distortion.

Adaptive optics uses a two-step process to create clearer images. First, since every sample is different, Shroff's team measured how a particular sample distorts the light. This information is then used to create a clear image by adjusting a deformable mirror. A key component of the method is two-photon microscopy, used to generate a small point of light deep inside the sample. By moving this light throughout the sample and collecting information on how it is being distorted, Shroff and his team are able to adjust the shape of the mirror to cancel out the distortions, thus creating a clear image of the whole sample.

This new microscope can be added to the arsenal of tools that Shroff's lab has developed over the years. (Read more about some of his other advances here.) Most of Shroff's improvements on microscopy technology have been focused on giving scientists the ability to see biological samples ever more clearly in their native 3D environment. This is important for researchers because, as Shroff said, "Life did not evolve on a coverslip." "We have been able to view cell biology at high resolution on a microscope slide for a long time," said Shroff, "but many times that's not how those cells exist in nature."

The unique aspects of this microscope continue to expand the efficacy of super-resolution microscopy. Shroff and his team are already looking at new ways to use this combination of technologies to create clearer images more quickly. "Efficiency and speed are key," emphasized Shroff. "The faster we can image live samples and the less we can interfere with their environment, the better we can understand how biology truly works."


Story Source:

Materials provided by National Institute of Biomedical Imaging and Bioengineering. Note: Content may be edited for style and length.


Journal Reference:

  1. Wei Zheng, Yicong Wu, Peter Winter, Robert Fischer, Damian Dalle Nogare, Amy Hong, Chad McCormick, Ryan Christensen, William P Dempsey, Don B Arnold, Joshua Zimmerberg, Ajay Chitnis, James Sellers, Clare Waterman, Hari Shroff. Adaptive optics improves multiphoton super-resolution imaging. Nature Methods, 2017; DOI: 10.1038/nmeth.4337

Cite This Page:

National Institute of Biomedical Imaging and Bioengineering. "New microscope uses adaptable mirror to create clearer images." ScienceDaily. ScienceDaily, 30 June 2017. <www.sciencedaily.com/releases/2017/06/170630090352.htm>.
National Institute of Biomedical Imaging and Bioengineering. (2017, June 30). New microscope uses adaptable mirror to create clearer images. ScienceDaily. Retrieved April 22, 2024 from www.sciencedaily.com/releases/2017/06/170630090352.htm
National Institute of Biomedical Imaging and Bioengineering. "New microscope uses adaptable mirror to create clearer images." ScienceDaily. www.sciencedaily.com/releases/2017/06/170630090352.htm (accessed April 22, 2024).

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