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New method enables easier and faster detection of celiac disease antibodies

Date:
November 26, 2019
Source:
University of Helsinki
Summary:
Researchers have developed a novel diagnostic method for the rapid on-site measurement of antibodies from patient samples. Now they have applied this new method for the diagnostics of celiac disease, with promising results. These results may prompt the development of similar tests for the diagnostics of other autoimmune disorders.
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Point-of-care (POC) testing is a rapidly growing sector, bringing medical testing from central laboratories to where the patient is receiving care.

Researchers from the University of Helsinki have developed a novel diagnostic method coined RFS (Rapid FRET serodiagnostics) for the rapid on-site measurement of antibodies from patient samples. This technology could revolutionize the serodiagnosis of microbial, autoimmune and allergic disorders.

Now this team of researchers have applied the new concept for diagnostics of celiac disease.

MOST PATIENTS ARE UNAWARE OF THEIR CELIAC DISEASE

Celiac disease is an autoimmune disorder typically presenting as non-specific gastrointestinal symptoms. It affects approximately 1 % of world population, with more than 90 % of patients undiagnosed. Even in affluent countries, more than half of celiacs are unaware of their disease. Delayed diagnosis is associated with impaired quality of life and persistent symptoms even after treatment.

Screening of celiac disease is recommended for patients with e.g. gastrointestinal symptoms or other autoimmune diseases as well as for the first-degree relatives of celiac patients. The screening test involves measurement of disease-specific antibodies from a blood sample.

EASY AND FASTER DETECTION OF CELIAC DISEASE ANTIBODIES

The researchers gathered samples from 70 celiacs at the Kuopio University Hospital, half from children and half from adults. Control samples from healthy donors were also collected. The samples were measured using the new assay and results compared with two currently used methods.

"The performance of the test was comparable to that of current methods. The prevailing method involves transporting the sample to a central laboratory and a multi-step procedure taking hours. With the new method, results can be achieved in less than half an hour by simply combining the sample and a reagent mix, waiting for a while and reading the result.," Juuso Rusanen, MD, explains. He continues:

"We hope our rapid method could lower the threshold for screening of celiac disease and thus help overcome the vast underdiagnosis of this relatively common condition."

"Additionally, this is the first time the new method has been used for diagnostics of autoimmune disease. This is a promising result, and prompts the development of similar tests for diagnostics of other autoimmune disorders," Rusanen points out.


Story Source:

Materials provided by University of Helsinki. Note: Content may be edited for style and length.


Journal Reference:

  1. Juuso Rusanen, Anne Toivonen, Jussi Hepojoki, Satu Hepojoki, Pekka Arikoski, Markku Heikkinen, Outi Vaarala, Jorma Ilonen, Klaus Hedman. LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection. PLOS ONE, 2019; 14 (11): e0225851 DOI: 10.1371/journal.pone.0225851

Cite This Page:

University of Helsinki. "New method enables easier and faster detection of celiac disease antibodies." ScienceDaily. ScienceDaily, 26 November 2019. <www.sciencedaily.com/releases/2019/11/191126155508.htm>.
University of Helsinki. (2019, November 26). New method enables easier and faster detection of celiac disease antibodies. ScienceDaily. Retrieved March 28, 2024 from www.sciencedaily.com/releases/2019/11/191126155508.htm
University of Helsinki. "New method enables easier and faster detection of celiac disease antibodies." ScienceDaily. www.sciencedaily.com/releases/2019/11/191126155508.htm (accessed March 28, 2024).

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