Featured Research

from universities, journals, and other organizations

New Technique Detects Protein Changes With High Sensitivity And Selectivity

Date:
September 14, 2007
Source:
American Society for Biochemistry and Molecular Biology
Summary:
Scientists describe a new technique that can detect how proteins undergo changes inside a cell. The technique promises to improve our understanding of how proteins inside cells work and identify how some proteins are not modified properly in common diseases such as cancer and cardiovascular diseases.

Scientists describe a new technique that can detect how proteins undergo changes inside a cell. The technique promises to improve our understanding of how proteins inside cells work and identify how some proteins are not modified properly in common diseases such as cancer and cardiovascular diseases.

Related Articles


In 2006, Ola Soderberg and colleagues established a technique called in situ proximity ligation assay (in situ PLA) to reveal protein-protein interactions in cells. The technique recognizes a target protein by binding a "probe" consisting of a pair of proteins attached to DNA onto the target protein.

Then the DNA is replicated, producing a molecule that can be visualized under a microscope as a fluorescent spot -- thus marking the presence of individual molecules in the target protein.

In the new study, Soderberg and colleagues developed a generalized version of the technique in which different probes can identify proteins that have undergone various changes in their structure. The researchers used this technique to detect a protein on the membrane of cells called platelet-derived growth factor receptor beta, which undergoes changes that will promote cell proliferation and movement.

The technique is more sensitive and selective than other currently-used techniques, that is, it does not miss as many proteins as the other techniques do and the rate of mix-ups among the detected proteins is lower.

Article: "In Situ Detection of Phosphorylated Platelet-derived Growth Factor Receptor Beta Using a Generalized Proximity Ligation Method," by Malin Jarvius, Janna Paulsson, Irene Weibrecht, Karl-Johan Leuchowius, Ann-Catrin Andersson, Carolina Wahlby, Mats Gullberg, Johan Botling, Tobias Sjoblom, Boyka Markova, Arne Ostman, Ulf Landegren, and Ola Soderberg, Molecular & Cellular Proteomics, Sept. 2007

.


Story Source:

The above story is based on materials provided by American Society for Biochemistry and Molecular Biology. Note: Materials may be edited for content and length.


Cite This Page:

American Society for Biochemistry and Molecular Biology. "New Technique Detects Protein Changes With High Sensitivity And Selectivity." ScienceDaily. ScienceDaily, 14 September 2007. <www.sciencedaily.com/releases/2007/09/070912185951.htm>.
American Society for Biochemistry and Molecular Biology. (2007, September 14). New Technique Detects Protein Changes With High Sensitivity And Selectivity. ScienceDaily. Retrieved March 30, 2015 from www.sciencedaily.com/releases/2007/09/070912185951.htm
American Society for Biochemistry and Molecular Biology. "New Technique Detects Protein Changes With High Sensitivity And Selectivity." ScienceDaily. www.sciencedaily.com/releases/2007/09/070912185951.htm (accessed March 30, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Monday, March 30, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Bionic Ants Could Be Tomorrow's Factory Workers

Bionic Ants Could Be Tomorrow's Factory Workers

Reuters - Innovations Video Online (Mar. 30, 2015) Industrious 3D printed bionic ants working together could toil in the factories of the future, says German technology company Festo. The robotic insects cooperate and coordinate their actions and movements to achieve a common aim. Amy Pollock reports. Video provided by Reuters
Powered by NewsLook.com
Captive-Born Panda Triplets Are Eight Months Old

Captive-Born Panda Triplets Are Eight Months Old

Reuters - Light News Video Online (Mar. 30, 2015) The world&apos;s only surviving captivity-born panda triplets turn eight months old, according to China’s state media. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Lions Make Surprise Comeback in Gabon

Lions Make Surprise Comeback in Gabon

AFP (Mar. 30, 2015) Lions have made a comeback in southeast Gabon, after disappearing for years, according to live footage from US wildlife organisation Panthera. Duration: 00:32 Video provided by AFP
Powered by NewsLook.com
Ancient Egyptian Beer Making Vessels Discovered in Israel

Ancient Egyptian Beer Making Vessels Discovered in Israel

AFP (Mar. 30, 2015) Fragments of pottery used by Egyptians to make beer and dating back 5,000 years have been discovered on a building site in Tel Aviv, the Israeli Antiquities Authority said on Sunday. Duration: 00:51 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins