Featured Research

from universities, journals, and other organizations

New Technique Detects Protein Changes With High Sensitivity And Selectivity

Date:
September 14, 2007
Source:
American Society for Biochemistry and Molecular Biology
Summary:
Scientists describe a new technique that can detect how proteins undergo changes inside a cell. The technique promises to improve our understanding of how proteins inside cells work and identify how some proteins are not modified properly in common diseases such as cancer and cardiovascular diseases.

Scientists describe a new technique that can detect how proteins undergo changes inside a cell. The technique promises to improve our understanding of how proteins inside cells work and identify how some proteins are not modified properly in common diseases such as cancer and cardiovascular diseases.

Related Articles


In 2006, Ola Soderberg and colleagues established a technique called in situ proximity ligation assay (in situ PLA) to reveal protein-protein interactions in cells. The technique recognizes a target protein by binding a "probe" consisting of a pair of proteins attached to DNA onto the target protein.

Then the DNA is replicated, producing a molecule that can be visualized under a microscope as a fluorescent spot -- thus marking the presence of individual molecules in the target protein.

In the new study, Soderberg and colleagues developed a generalized version of the technique in which different probes can identify proteins that have undergone various changes in their structure. The researchers used this technique to detect a protein on the membrane of cells called platelet-derived growth factor receptor beta, which undergoes changes that will promote cell proliferation and movement.

The technique is more sensitive and selective than other currently-used techniques, that is, it does not miss as many proteins as the other techniques do and the rate of mix-ups among the detected proteins is lower.

Article: "In Situ Detection of Phosphorylated Platelet-derived Growth Factor Receptor Beta Using a Generalized Proximity Ligation Method," by Malin Jarvius, Janna Paulsson, Irene Weibrecht, Karl-Johan Leuchowius, Ann-Catrin Andersson, Carolina Wahlby, Mats Gullberg, Johan Botling, Tobias Sjoblom, Boyka Markova, Arne Ostman, Ulf Landegren, and Ola Soderberg, Molecular & Cellular Proteomics, Sept. 2007

.


Story Source:

The above story is based on materials provided by American Society for Biochemistry and Molecular Biology. Note: Materials may be edited for content and length.


Cite This Page:

American Society for Biochemistry and Molecular Biology. "New Technique Detects Protein Changes With High Sensitivity And Selectivity." ScienceDaily. ScienceDaily, 14 September 2007. <www.sciencedaily.com/releases/2007/09/070912185951.htm>.
American Society for Biochemistry and Molecular Biology. (2007, September 14). New Technique Detects Protein Changes With High Sensitivity And Selectivity. ScienceDaily. Retrieved November 24, 2014 from www.sciencedaily.com/releases/2007/09/070912185951.htm
American Society for Biochemistry and Molecular Biology. "New Technique Detects Protein Changes With High Sensitivity And Selectivity." ScienceDaily. www.sciencedaily.com/releases/2007/09/070912185951.htm (accessed November 24, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Monday, November 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Stray Dog Follows Adventure Racing Team for 6-Day Endurance Race

Stray Dog Follows Adventure Racing Team for 6-Day Endurance Race

Buzz60 (Nov. 24, 2014) A Swedish Adventure racing team travels to try and win a world title, but comes home with something way better: a stray dog that joined the team for much of the grueling 430-mile race. Jen Markham has the story. Video provided by Buzz60
Powered by NewsLook.com
Red Panda Cubs Explore the Bratislava Zoo

Red Panda Cubs Explore the Bratislava Zoo

AFP (Nov. 24, 2014) Four-month old Red Panda twins Pim and Pam still rely on their mother for breast milk at the Bratislava Zoo in Slovakia, but the precocious cubs have begun to branch out to solid foods, as well. Duration: 00:41 Video provided by AFP
Powered by NewsLook.com
Are Female Bosses More Likely To Be Depressed?

Are Female Bosses More Likely To Be Depressed?

Newsy (Nov. 24, 2014) A new study links greater authority with increased depressive symptoms among women in the workplace. Video provided by Newsy
Powered by NewsLook.com
Winter Can Cause Depression — Here's How To Combat It

Winter Can Cause Depression — Here's How To Combat It

Newsy (Nov. 23, 2014) Millions of American suffer from seasonal depression every year. It can lead to adverse health effects, but there are ways to ease symptoms. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins