Featured Research

from universities, journals, and other organizations

Adjusting to high temperatures: Researchers discover multifunctional enzyme active in metabolism

Date:
October 11, 2011
Source:
Albert-Ludwigs-Universität Freiburg
Summary:
Gluconeogenesis is the ability to re-synthesize sugar out of simpler chemical building blocks. It is a central pathway of the metabolism in humans as well as simple bacteria. Researchers have been unable to scientifically analyze this conclusively until now. Scientists in Germany have described a fundamentally new type of multifunctional enzyme in this metabolic pathway.

Illustration of different states of fructose-1,6-bisphosphate aldolase/phosphatase: native enzyme (A) and open chain form (B).
Credit: Image courtesy of Albert-Ludwigs-Universität Freiburg

Gluconeogenesis is the ability to re-synthesize sugar out of simpler chemical building blocks. It is a central pathway of the metabolism in humans as well as simple bacteria. Researchers have been unable to scientifically analyse this conclusively until now.

Related Articles


Together with the research groups led by Prof. Dr. Oliver Einsle at the Institute of Organic Chemistry and Biochemistry and Prof. Dr. Georg Fuchs from the Institute of Biology II, research groups at the Centre for Biological Signalling Studies (BIOSS), the Spemann Graduate School of Biology and Medicine (SGBM) and the Freiburg Institute of Advanced Studies (FRIAS) have described a fundamentally new type of multifunctional enzyme in this metabolic pathway.

Their new findings have now been published online in the current issue of the journal Nature.

The researchers discovered that the entire field of archaea organisms, which are so-called ancient bacteria, lacks aldolase, which is a key enzyme in gluconeogenesis. In 2010, Dr. Rafael Say and Dr. Georg Fuchs discovered that the enzyme fructose-1,6-bisphosphate can also perform the functions of aldolase, even though the enzyme’s amino acids involved in the aldolase reaction are far removed from the binding site of the substrate.

Dr. Juan Du and Dr. Wei Lü have researched the enzyme fructose-1,6-bisphosphate aldolase/phosphatase (FBPAP) in Prof. Dr. Oliver Einsle’s research group. Using high resolution imaging, they were able to determine three-dimensional structures that revealed a surprisingly complex reaction mechanism in detail. They discovered that it is the actual protein that reorganizes itself around the tightly bound substrate molecule. The entire complex process is controlled by the binding of additional magnesium ions, which are abundantly available in the cell. The binding of these ions completely changes the enzyme in three areas, and its active centre is transformed from an aldolase to a phosphatase.

The researchers from the University of Freiburg characterized this mechanism as a new, multifunctional enzyme. They suspect that similar additional catalytic properties can be found in other proteins but these have not yet been discovered. Regarding FBPAP, this unusual mechanism is believed to be an adjustment to living in higher temperatures, where the starting substances of reaction are instable.


Story Source:

The above story is based on materials provided by Albert-Ludwigs-Universität Freiburg. Note: Materials may be edited for content and length.


Journal Reference:

  1. Juan Du, Rafael F. Say, Wei Lü, Georg Fuchs, Oliver Einsle. Active-site remodelling in the bifunctional fructose-1,6-bisphosphate aldolase/phosphatase. Nature, 2011; DOI: 10.1038/nature10458

Cite This Page:

Albert-Ludwigs-Universität Freiburg. "Adjusting to high temperatures: Researchers discover multifunctional enzyme active in metabolism." ScienceDaily. ScienceDaily, 11 October 2011. <www.sciencedaily.com/releases/2011/10/111011074829.htm>.
Albert-Ludwigs-Universität Freiburg. (2011, October 11). Adjusting to high temperatures: Researchers discover multifunctional enzyme active in metabolism. ScienceDaily. Retrieved November 27, 2014 from www.sciencedaily.com/releases/2011/10/111011074829.htm
Albert-Ludwigs-Universität Freiburg. "Adjusting to high temperatures: Researchers discover multifunctional enzyme active in metabolism." ScienceDaily. www.sciencedaily.com/releases/2011/10/111011074829.htm (accessed November 27, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Thursday, November 27, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

New Dinosaur Species Found in Museum Collection

New Dinosaur Species Found in Museum Collection

Reuters - Innovations Video Online (Nov. 27, 2014) — A British palaeontologist has discovered a new species of dinosaur while studying fossils in a Canadian museum. Pentaceratops aquilonius was related to Triceratops and lived at the end of the Cretaceous Period, around 75 million years ago. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Classic Hollywood Memorabilia Goes Under the Hammer

Classic Hollywood Memorabilia Goes Under the Hammer

Reuters - Entertainment Video Online (Nov. 26, 2014) — The iconic piano from "Casablanca" and the Cowardly Lion suit from "The Wizard of Oz" fetch millions at auction. Sara Hemrajani reports. Video provided by Reuters
Powered by NewsLook.com
Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) — Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

Newsy (Nov. 25, 2014) — The US FDA is announcing new calorie rules on Tuesday that will require everywhere from theaters to vending machines to include calorie counts. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins