Featured Research

from universities, journals, and other organizations

The 'gold' standard: A rapid, cheap method of detecting dengue virus

Date:
June 28, 2013
Source:
BioMed Central
Summary:
Researchers are reporting the development of an easy to use, low cost method of detecting dengue virus in mosquitoes based on gold nanoparticles. The assay is able to detect lower levels of the virus than current tests, and is easy to transport and use in remote regions.

The development of an easy to use, low cost method of detecting dengue virus in mosquitoes based on gold nanoparticles is reported in BioMed Central's open access journal Virology Journal. The assay is able to detect lower levels of the virus than current tests, and is easy to transport and use in remote regions.

Related Articles


Half the world's population is at risk of Dengue virus infection -- it infects 50-100 million people per year, approximately half a million of these require hospitalization and 2.5% (most of which are children) will die. It is one of the most dangerous viruses in the world with no vaccine, and it does not respond to antiviral therapy. The main method of controlling infection remains destruction of the standing water where the mosquitoes, which transmit the virus to people, breed.

It is consequently vitally important to have a way of determining if mosquitoes are carrying Dengue virus, which can be used on site and that does not require specialist equipment.

Researchers from the University of Notre Dame, USA, used a DNAzyme linked to gold nanoparticles which recognises a short sequence of the viral RNA genome common to all four types of Dengue. Once bound, adding magnesium and heating to 37C causes the DNAZyme to cut the RNA leaving the gold nanoparticles free to clump together. This aggregation can be easily seen as a red to clear/colourless colour change.

The components of this test are stable at temperatures above 30C which means that they are easy to store and transport and the assay is able to detect as little as 10 viruses in each sample containing 10-20 mosquitoes.

The ultimate goal is to detect virus infection in just a single infected mosquito or cell. Dr James Carter, the lead author of this study explained, "Full development of our novel DDZ-AuNP detection method will provide a practical, rapid, and low cost alternative for the detection of DENV in mosquito cells and tissues, and possibly infected patient serum, in a matter of minutes with little to no specialized training required."


Story Source:

The above story is based on materials provided by BioMed Central. Note: Materials may be edited for content and length.


Journal Reference:

  1. James R Carter, Velmurugan Balaraman, Cheryl A Kucharski, Tresa S Fraser, Malcolm J Fraser. A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches. Virology Journal, 2013; 10 (1): 201 DOI: 10.1186/1743-422X-10-201

Cite This Page:

BioMed Central. "The 'gold' standard: A rapid, cheap method of detecting dengue virus." ScienceDaily. ScienceDaily, 28 June 2013. <www.sciencedaily.com/releases/2013/06/130628092153.htm>.
BioMed Central. (2013, June 28). The 'gold' standard: A rapid, cheap method of detecting dengue virus. ScienceDaily. Retrieved January 30, 2015 from www.sciencedaily.com/releases/2013/06/130628092153.htm
BioMed Central. "The 'gold' standard: A rapid, cheap method of detecting dengue virus." ScienceDaily. www.sciencedaily.com/releases/2013/06/130628092153.htm (accessed January 30, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, January 30, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Nanoscale Sensor Could Help Wine Producers and Clinical Scientists

Nanoscale Sensor Could Help Wine Producers and Clinical Scientists

Reuters - Innovations Video Online (Jan. 30, 2015) A nanosensor that mimics the oral effects and sensations of drinking wine has been developed by Danish and Portuguese researchers. Jim Drury saw it in operation. Video provided by Reuters
Powered by NewsLook.com
Discovery Of 'Dragon' Dinosaur In China Could Explain Myths

Discovery Of 'Dragon' Dinosaur In China Could Explain Myths

Newsy (Jan. 30, 2015) A long-necked dinosaur from the Jurassic Period was discovered in China. Researchers think it could answer mythology questions. Video provided by Newsy
Powered by NewsLook.com
Poll Says Firstborn Is Responsible, Youngest Is Funnier

Poll Says Firstborn Is Responsible, Youngest Is Funnier

Newsy (Jan. 30, 2015) According to a poll out of the U.K., eldest siblings feel more responsible and successful than their younger siblings. Video provided by Newsy
Powered by NewsLook.com
Brawling Pandas Are Violently Adorable

Brawling Pandas Are Violently Adorable

Buzz60 (Jan. 29, 2015) Video of pandas play fighting at the Chengdu Research Base in China will make your day. Mara Montalbano (@maramontalbano) shows us. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins