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Novel technique for finding drugs to combat malaria developed

Date:
February 10, 2015
Source:
Albert Einstein College of Medicine of Yeshiva University
Summary:
Each year nearly 600,000 people--mostly children under age five and pregnant women in sub-Saharan Africa--die from malaria, caused by single-celled parasites that grow inside red blood cells. The most deadly malarial species--Plasmodium falciparum--has proven notoriously resistant to treatment efforts. But thanks to a novel approach, researchers can readily screen thousands of drugs to find those potentially able to kill P. falciparum.
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Each year nearly 600,000 people -- mostly children under age five and pregnant women in sub-Saharan Africa -- die from malaria, caused by single-celled parasites that grow inside red blood cells. The most deadly malarial species -- Plasmodium falciparum -- has proven notoriously resistant to treatment efforts. But thanks to a novel approach developed by scientists at Albert Einstein College of Medicine of Yeshiva University and described in the January 20 online edition of ACS Chemical Biology, researchers can readily screen thousands of drugs to find those potentially able to kill P. falciparum.

Scientists have known for more than a decade that malaria parasites have an Achilles heel: Like all cells, they require two key building blocks -- purines and pyrimidines -- to synthesize their DNA and RNA. But malaria parasites can't synthesize purines on their own. Instead, they must import purines from the host red blood cells that they invade. A parasite protein called PfENT1 transports purines from blood cell into the parasites. So drugs that block PfENT1 could conceivably kill the parasites by depriving them of purines they need -- but an experimental approach for identifying PfENT1 inhibitors didn't exist, until now.

Einstein's Myles Akabas, M.D., Ph.D., developed a novel yeast-based high-throughput assay for identifying inhibitors of the PfENT1 transporter. Dr. Akabas worked with two MSTP students in his lab (I.J. Frame and Roman Deniskin) as well as colleagues at Einstein (Drs. Ian Willis and Robyn Moir) and Columbia University (Drs. Donald Landry and David Fidock). The researchers used their technique to screen 64,560 different compounds. They identified 171 potential antimalarial drugs. Studies of nine of the most potent drugs showed that they kill P. falciparum parasites in laboratory culture.

"We've shown that the PfENT1 transporter is a potential drug target for developing novel antimalarial drugs," said Dr. Akabas, senior author of the ACS Chemical Biology paper and a professor of physiology & biophysics, of medicine and in the Dominick P. Purpura Department of Neuroscience at Einstein. "By using our rather simple approach, scientists could create similar high-throughput screens to identify inhibitors for killing other parasites that rely on transporters to import essential nutrients."

Last month, the National Institutes of Health awarded Dr. Akabas and his Columbia University collaborators a five-year, $3.45-million grant (1R01AI116665) to use his high-throughput assay to find and develop antimalarial drugs. Einstein has applied for patents to cover this assay.


Story Source:

Materials provided by Albert Einstein College of Medicine of Yeshiva University. Note: Content may be edited for style and length.


Journal Reference:

  1. I. J. Frame, Roman Deniskin, Alison Rinderspacher, Francine Katz, Shi-Xian Deng, Robyn D. Moir, Sophie H. Adjalley, Olivia Coburn-Flynn, David A. Fidock, Ian M. Willis, Donald W. Landry, Myles H. Akabas. Yeast-Based High-Throughput Screen IdentifiesPlasmodium falciparumEquilibrative Nucleoside Transporter 1 Inhibitors That Kill Malaria Parasites. ACS Chemical Biology, 2015; 150130135621003 DOI: 10.1021/cb500981y

Cite This Page:

Albert Einstein College of Medicine of Yeshiva University. "Novel technique for finding drugs to combat malaria developed." ScienceDaily. ScienceDaily, 10 February 2015. <www.sciencedaily.com/releases/2015/02/150210210729.htm>.
Albert Einstein College of Medicine of Yeshiva University. (2015, February 10). Novel technique for finding drugs to combat malaria developed. ScienceDaily. Retrieved December 5, 2024 from www.sciencedaily.com/releases/2015/02/150210210729.htm
Albert Einstein College of Medicine of Yeshiva University. "Novel technique for finding drugs to combat malaria developed." ScienceDaily. www.sciencedaily.com/releases/2015/02/150210210729.htm (accessed December 5, 2024).

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