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Mitochondria altered in human cell model of Parkinson's disease

Date:
April 2, 2015
Source:
Buck Institute for Age Research
Summary:
Based on research in fruit flies, it has long been suspected that the most common mutation linked to both sporadic and familial Parkinson's disease (PD) wreaks its havoc by altering the function of mitochondria in neurons that produce the neurotransmitter dopamine. Using stem cells derived from patients who have PD, scientists have confirmed that finding in human cells for the first time.
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Based on research in fruit flies, it has long been suspected that the most common mutation linked to both sporadic and familial Parkinson's disease (PD) wreaks its havoc by altering the function of mitochondria in neurons that produce the neurotransmitter dopamine. Using stem cells derived from patients who have PD, scientist at the Buck Institute have confirmed that finding in human cells for the first time. In research published in the April 2nd early online edition of Stem Cell Reports, Buck researchers also provide a valuable tool for testing potential treatments for the progressive, incurable neurodegenerative disease.

"If we can find existing drugs or develop new ones that prevent damage to the mitochondria we would have a potential treatment for PD," said Buck faculty Xianmin Zeng, PhD, lead scientist on the study. "This is the first time we can show a real and observable change in mitochondria in human cells affected by a PD mutation," she said.

Mitochondria are energy-producing organelles found in large numbers in most cells and are necessary for life. Zeng says the Park2 mutation altered mitochondrial structure and function in dopamine producing neurons, causing them to die. Dopamine is an essential neurotransmitter which regulates movement and emotion.

The research involved making mutations in the Park2 gene in well-characterized induced pluripotent stem cell (iPSC) lines as well as generating iPSC lines from skin cells of four patients who have PD. "What we saw was extraordinary," said Zeng. "We saw the same characteristics in the dopaminergic neurons derived from patients as well as ones derived from the lines where the mutation was introduced."

The finding promises to be significant in the search for treatments for an incurable disease, which affects nearly one million Americans and causes a progressive decline in movement and muscle control. Existing drugs can ease symptoms but often cause troubling side effects over time. While using patient-specific iPSC lines has been held as a potential gold standard for testing potential therapeutics for PD, Zeng says there has always been concern that background genetic differences between patients would render the testing inconclusive for larger populations of PD patients. According to Zeng, this research addresses those concerns and paves the way for testing of compounds for PD in human cells, an activity that is underway in the Buck lab of Julie Andersen, PhD, who also studies PD.

Zeng, who receives support from the California Institute for Regenerative Medicine (CIRM), says there are up to ten mutations linked to PD. Her lab is developing human iPSC models based on mutations in Pink1, SNCA, Park7 and Lrrk2, which are those more closely implicated in PD. Zeng is also involved in research aimed at getting a stem cell derived treatment for PD ready for testing in humans.


Story Source:

Materials provided by Buck Institute for Age Research. Note: Content may be edited for style and length.


Journal Reference:

  1. Atossa Shaltouki et al. Mitochondrial alterations by Parkin in dopaminergic neurons using Park2 patient-specific and Park2 knock-out isogenic iPSC lines. Stem Cell Reports, April 2015 DOI: 10.1016/j.stemcr.2015.02.019

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Buck Institute for Age Research. "Mitochondria altered in human cell model of Parkinson's disease." ScienceDaily. ScienceDaily, 2 April 2015. <www.sciencedaily.com/releases/2015/04/150402132741.htm>.
Buck Institute for Age Research. (2015, April 2). Mitochondria altered in human cell model of Parkinson's disease. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2015/04/150402132741.htm
Buck Institute for Age Research. "Mitochondria altered in human cell model of Parkinson's disease." ScienceDaily. www.sciencedaily.com/releases/2015/04/150402132741.htm (accessed May 23, 2017).

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