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Bacterial protein serves as sensor

Date:
June 10, 2015
Source:
Albert-Ludwigs-Universität Freiburg
Summary:
A method capable of precisely measuring the size of individual molecules has been developed by scientists. To do this, the researchers used the protein aerolysin from the bacterium Aeromonas hydrophila instead of the protein previously used for this purpose, alpha-hemolysin from the bacterium Staphylococcus aureus.
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Principle of size-discrimination of molecules with bacterial nanopores: When a molecule is placed in the pore formed by the protein (above center), a part of the current (red trail) is briefly interrupted by the open pore (above left). Larger chain molecules block the current more completely and for longer periods of time than smaller ones. This effect is used to measure the size of the molecules.
Credit: Illustration: Jan C. Behrends

A German-French team led by Prof. Dr. Jan C. Behrends and Dr. Gerhard Baaken from the University of Freiburg and Dr. Abdelghani Oukhaled from the Universities of Evry and Cergy-Pontoise has developed a method capable of precisely measuring the size of individual molecules.

To do so, the researchers used the protein aerolysin from the bacterium Aeromonas hydrophila instead of the protein previously used for this purpose, alpha-hemolysin from the bacterium Staphylococcus aureus. The method remains unchanged: The protein forms a pore in an artificial cell membrane.

The researchers insert the molecule whose size they wish to measure into this pore by guiding an ionic current through it. The molecule partially blocks this current -- similar to the way an object illuminated by a spotlight throws a shadow. The rest of the ionic current, which makes it through the pore, can then be used to measure the molecule.

"The new pore is much more suitable for determining the entire size range of molecules," says Behrends. The researchers published their findings in the journal ACS Nano.

The key advantage of the new method is that polymers -- chain molecules composed of repeating elements -- remain in the new pore much longer than a millisecond, while they only stay in the hemolysin pore for less than a millisecond.

The method allows scientists to determine the difference in size between two molecules that only differ with regard to a single link of the chain. Polymers like the water-soluble, non-toxic, and non-allergenic polyethylene glycol have a wide range of uses in medicine and biotechnology.

For instance, they can enhance the stability of medicinal agents. This requires precise information about how large the links of the chain in the molecule are and how they are distributed.

The new and improved method is capable of providing this information -- even in the case of short chains whose size was nearly impossible to discriminate with the pore previously used for this purpose. "The technical feasibility of this method for determining the size of water-soluble polymers is thus now within reach," says Behrends.


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Materials provided by Albert-Ludwigs-Universität Freiburg. Note: Content may be edited for style and length.


Journal Reference:

  1. Gerhard Baaken, Ibrahim Halimeh, Laurent Bacri, Juan Pelta, Abdelghani Oukhaled, Jan C. Behrends. High-Resolution Size-Discrimination of Single Nonionic Synthetic Polymers with a Highly Charged Biological Nanopore. ACS Nano, 2015; 150609141925007 DOI: 10.1021/acsnano.5b02096

Cite This Page:

Albert-Ludwigs-Universität Freiburg. "Bacterial protein serves as sensor." ScienceDaily. ScienceDaily, 10 June 2015. <www.sciencedaily.com/releases/2015/06/150610093217.htm>.
Albert-Ludwigs-Universität Freiburg. (2015, June 10). Bacterial protein serves as sensor. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2015/06/150610093217.htm
Albert-Ludwigs-Universität Freiburg. "Bacterial protein serves as sensor." ScienceDaily. www.sciencedaily.com/releases/2015/06/150610093217.htm (accessed May 23, 2017).

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