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Structure of protein complex that plays key role in modulating immune system revealed

Search for new inflammatory disease drugs may be aided by detailed structure of distinct ubiquitin ligase complex

Date:
January 20, 2016
Source:
Sanford-Burnham Prebys Medical Discover Institute
Summary:
The search for new inflammatory disease drugs may be aided by a detailed structure of distinct ubiquitin ligase complex.
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This image shows E3 ubiquitin ligase (green), E2 ubiquitin enzyme (orange), 'activated ubiquitin' (cyan), and 'allosteric ubiquitin' (blue).
Credit: Bernhard Lechtenberg

Scientists at Sanford Burnham Prebys Medical Discovery Institute (SBP) have solved the atomic structure of a unique ubiquitin ligase complex. Ubiquitin is best known for its role in protein degradation, but more recently seen as important for cell signaling, DNA repair, anti-inflammatory, and immune responses.

The study, published today in Nature, opens the door for developing a novel class of drug targets for cancer as well as inflammatory diseases such as rheumatoid arthritis, Crohn's disease and psoriasis.

"Our new research revealing the fully active structure of an RBR E3 ligase holds significant therapeutic potential in oncology and immunology," said Bernhard Lechtenberg, Ph.D., postdoctoral fellow at SBP and lead author of the study. "The three-dimensional structure we present provides detailed insights into the molecular architecture of the complex and allows us to draw conclusions about how it works."

Ubiquitin is a small protein that helps regulate the function of other proteins in the body. E3 ligases carry out a process known as ubiquitination, where ubiquitin is attached to a substrate protein, changing the way it functions.

There are three classes of E3 ligases, but members of the RBR type have most recently emerged as a novel and relatively untapped class of targets for drug discovery because of their role in modulating the immune system.

"We were surprised to find how the active form of the E3 ligase we analyzed, called HOIP, attaches ubiquitin in a markedly different way--an elongated fashion--compared to the other types of E3 ligases," said Stefan Riedl, Ph.D., associate professor in SBP's NCI-designated Cancer Center and senior author of the paper. "This may be key to its role in activating the NF-kB pathway, a signaling process that is well established as a regulator of cell survival and death, and helps coordinate the immune system.

"NF-kB is the master regulator of inflammation inside cells, and its activation is believed to promote cancer development by inhibiting cell death and promoting inflammation. This study removes a significant technical barrier that has prevented exploiting RBR E3 ligases as a drug target for cancer and inflammatory disorders.

"Our next step is to continue to work very closely with our biology and immunology collaborators to more fully understand the regulation of RBR E3 ligases," added Riedl.

The study was performed in collaboration with the Argonne National Laboratory and the University of Otago, New Zealand.

The study was supported by NIH grant R01AA017238, and EMBO Long-term Postdoctoral Fellowship (Bernhard Lechtenberg), a Rutherford Discovery Fellowship from New Zealand, and NCI grant P30CA030199.


Story Source:

Materials provided by Sanford-Burnham Prebys Medical Discover Institute. Original written by Jessica Moore. Note: Content may be edited for style and length.


Journal Reference:

  1. Bernhard C. Lechtenberg, Akhil Rajput, Ruslan Sanishvili, Małgorzata K. Dobaczewska, Carl F. Ware, Peter D. Mace, Stefan J. Riedl. Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation. Nature, 2016; DOI: 10.1038/nature16511

Cite This Page:

Sanford-Burnham Prebys Medical Discover Institute. "Structure of protein complex that plays key role in modulating immune system revealed: Search for new inflammatory disease drugs may be aided by detailed structure of distinct ubiquitin ligase complex." ScienceDaily. ScienceDaily, 20 January 2016. <www.sciencedaily.com/releases/2016/01/160120141528.htm>.
Sanford-Burnham Prebys Medical Discover Institute. (2016, January 20). Structure of protein complex that plays key role in modulating immune system revealed: Search for new inflammatory disease drugs may be aided by detailed structure of distinct ubiquitin ligase complex. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2016/01/160120141528.htm
Sanford-Burnham Prebys Medical Discover Institute. "Structure of protein complex that plays key role in modulating immune system revealed: Search for new inflammatory disease drugs may be aided by detailed structure of distinct ubiquitin ligase complex." ScienceDaily. www.sciencedaily.com/releases/2016/01/160120141528.htm (accessed May 26, 2017).

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