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Link identified between nerve cell proteins and middle-age onset dementia

Date:
February 13, 2017
Source:
Nagoya University
Summary:
New research identifies a role for neuronal protein interaction in preventing frontotemporal lobar degeneration, a dementia that starts in middle age.
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Fused in sarcoma (FUS) and its binding partner proline- and glutamine-rich (SFPQ) regulates Mapt splicing, resulting balanced ratio of tau isoforms. Loss of interaction between FUS and SFPQ alters Mapt splicing, and subsequently increases 4-repeat tau/ 3-repeat tau ratio. This unbalanced tau isoform ratio leads to a neurodegenerative phenotype similar to FTLD.
Credit: © 2017 Yusuke Fujioka, Shinsuke Ishigaki, and Gen Sobue

Nagoya University-led research identifies role for neuronal protein interaction in preventing frontotemporal lobar degeneration, a dementia that starts in middle age.

Frontotemporal lobar degeneration (FTLD) is a type of dementia characterized by personality changes, language dysfunction, and abnormal behavior. It has an earlier onset than Alzheimer's disease, and is associated with a buildup of the tau protein in affected nerve cells (neurons).

Nagoya University-led Japanese research has now revealed that loss of the interaction between two RNA binding proteins changes the expression ratio of different forms of tau protein, producing the FTLD phenotype in mice, and that this could be rescued by rebalancing the tau ratio. The study was reported in Cell Reports.

The RNA binding protein FUS is linked to both familial and sporadic FTLD/ALS. The researchers investigated other proteins that bind the FUS complex within the nucleus and found another RNA metabolism regulator, SFPQ, to be key to the complex formation.

Both FUS and SFPQ control the process known as alternative splicing by which exons of a gene are joined to other exons or skipped altogether to produce different messenger RNAs and, consequently, different versions (isoforms) of the same protein. FUS/SFPQ-regulated alternative splicing of the Mapt gene at exon 10 produces two different tau isoforms (4R-T and 3R-T) that are usually balanced. However, the team showed that FUS or SFPQ silencing resulted in an excess of 4R-T over 3R-T.

The researchers generated mice lacking expression of FUS or SFPQ in a region of their brain important for memory and spatial navigation; the hippocampus. These mice were observed to have abnormal behaviors that resembled those of FTLD.

"They also had a reduced hippocampal volume, loss of neuronal cells, and less nerve cell growth than control animals," study first author Shinsuke Ishigaki says. "Crucially, the mice showed increased levels of a modified form of tau that is a known hallmark of FTLD and other neurodegenerative diseases."

The team attempted to rescue this disease phenotype in mice by rebalancing the 4R-T/3R-T ratio. "We achieved this by introducing a short sequence of RNA to block 4R-T expression," corresponding author Gen Sobue explains. "This recovered most of the changes associated with FTLD that had been caused by FUS or SFPQ silencing."

The researchers confirmed that the link between FUS/SFPQ and tau isoform regulation also exists in humans using a model from human stem cell-derived neurons and a mini-gene, implying a role for tau isoform imbalance in FTLD development in humans.


Story Source:

Materials provided by Nagoya University. Note: Content may be edited for style and length.


Journal Reference:

  1. Shinsuke Ishigaki, Yusuke Fujioka, Yohei Okada, Yuichi Riku, Tsuyoshi Udagawa, Daiyu Honda, Satoshi Yokoi, Kuniyuki Endo, Kensuke Ikenaka, Shinnosuke Takagi, Yohei Iguchi, Naruhiko Sahara, Akihiko Takashima, Hideyuki Okano, Mari Yoshida, Hitoshi Warita, Masashi Aoki, Hirohisa Watanabe, Haruo Okado, Masahisa Katsuno, Gen Sobue. Altered Tau Isoform Ratio Caused by Loss of FUS and SFPQ Function Leads to FTLD-like Phenotypes. Cell Reports, 2017; 18 (5): 1118 DOI: 10.1016/j.celrep.2017.01.013

Cite This Page:

Nagoya University. "Link identified between nerve cell proteins and middle-age onset dementia." ScienceDaily. ScienceDaily, 13 February 2017. <www.sciencedaily.com/releases/2017/02/170213090732.htm>.
Nagoya University. (2017, February 13). Link identified between nerve cell proteins and middle-age onset dementia. ScienceDaily. Retrieved March 23, 2017 from www.sciencedaily.com/releases/2017/02/170213090732.htm
Nagoya University. "Link identified between nerve cell proteins and middle-age onset dementia." ScienceDaily. www.sciencedaily.com/releases/2017/02/170213090732.htm (accessed March 23, 2017).