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SARS-CoV-2 spike mutation L452R evades human immune response and enhances infectivity, researchers find

Date:
July 20, 2021
Source:
Kumamoto University
Summary:
Researchers have shown that the L452R mutation of the SARS-CoV-2 spike protein, which is common to two variants (Epsilon and Delta), is involved in cellular immunity evasion via the human leukocyte antigen (HLA) A24, and enhances viral infectivity.
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FULL STORY

An international team of researchers led by Kumamoto and Tokyo Universities (Japan) have shown that the L452R mutation of the SARS-CoV-2 spike protein, which is common to two mutant strains (Epsilon and Delta), is involved in cellular immunity evasion via the human leukocyte antigen (HLA) A24, and enhances viral infectivity. HLA-A24 is one of the most prominent HLA-class I alleles, especially in East/Southeast Asian populations, which might make them particularly vulnerable to coronavirus variants with this mutation.

The ongoing novel coronavirus (SARS-CoV-2 or COVID-19) pandemic has, as of June 2021, infected over 150 million and killed over 3.5 million people worldwide. Vaccination drives around the world are currently underway, but there are still many unknowns, including the principles of infection pathogenesis, the principles viral replication, and the relationship between immune evasion and epidemic dynamics.

Acquired immunity can be broadly classified into humoral immunity mediated by neutralizing antibodies and cellular immunity mediated by helper and killer T cells. SARS-CoV-2 "variants of concern," such as the Alpha and Beta variants, have been studied worldwide for the possibility of humoral immunity evasion. However, cellular immunity evasion has not been reported.

In this study, the research group first used immunological experiments to demonstrate that an antigen derived from the SARS-CoV-2 spike protein is strongly recognized by HLA-A24-restricted cellular immunity, which is often found in Japanese people. They then performed a large-scale (>750,000) sequence analysis of SARS-CoV-2 strains and found several important mutations in the spike protein region typically recognized by HLA-A24. These are the Y453F spike mutations found in strain B.1.1.298, which was prevalent in Denmark in 2020, and the L452R mutation in B.1.427/429 and B.1.617 (commonly known as the Epsilon and Delta variants respectively) that are currently spreading around the world. Further immunological experiments demonstrated that these mutations escape HLA-A24 cellular immunity. The researchers believe that this is the first time a "variant of concern" has been demonstrated to evade cellular immunity.

The Y453F and L452R mutations were located in the receptor binding domain of the SARS-CoV-2 spike protein, which are crucial for gaining entry into host cells. Researchers thus examined the effects of these mutations on the infection and replication efficiency of the virus. They found that the L452R mutation enhances its membrane fusion activity, infectivity, and viral replication.

"The L452R mutation is a hallmark of the Delta variant that is currently spreading worldwide, and in Japan, about 60% of the population have HLA-A24, which is responsible for cellular immunity. The L452R mutation not only evades the HLA-A24 cellular immunity but can also enhance the infectivity of the virus," said the leader of immunology in the study, Dr. Chihiro Motozono." We have been carefully investigating the immune response against emerging SARS-CoV-2 variants in real time to monitor how the mutations affect human immunity and viral infectivity."

This research was posted in Cell Host & Microbe on 14 June 2021.


Story Source:

Materials provided by Kumamoto University. Note: Content may be edited for style and length.


Journal Reference:

  1. Chihiro Motozono, Mako Toyoda, Jiri Zahradnik, Akatsuki Saito, Hesham Nasser, Toong Seng Tan, Isaac Ngare, Izumi Kimura, Keiya Uriu, Yusuke Kosugi, Yuan Yue, Ryo Shimizu, Jumpei Ito, Shiho Torii, Akiko Yonekawa, Nobuyuki Shimono, Yoji Nagasaki, Rumi Minami, Takashi Toya, Noritaka Sekiya, Takasuke Fukuhara, Yoshiharu Matsuura, Gideon Schreiber, Terumasa Ikeda, So Nakagawa, Takamasa Ueno, Kei Sato. SARS-CoV-2 spike L452R variant evades cellular immunity and increases infectivity. Cell Host & Microbe, 2021; 29 (7): 1124 DOI: 10.1016/j.chom.2021.06.006

Cite This Page:

Kumamoto University. "SARS-CoV-2 spike mutation L452R evades human immune response and enhances infectivity, researchers find." ScienceDaily. ScienceDaily, 20 July 2021. <www.sciencedaily.com/releases/2021/07/210720114413.htm>.
Kumamoto University. (2021, July 20). SARS-CoV-2 spike mutation L452R evades human immune response and enhances infectivity, researchers find. ScienceDaily. Retrieved February 25, 2024 from www.sciencedaily.com/releases/2021/07/210720114413.htm
Kumamoto University. "SARS-CoV-2 spike mutation L452R evades human immune response and enhances infectivity, researchers find." ScienceDaily. www.sciencedaily.com/releases/2021/07/210720114413.htm (accessed February 25, 2024).

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