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Cellular bubbles used to deliver Parkinson's meds directly to brain

Date:
May 4, 2015
Source:
University of North Carolina at Chapel Hill
Summary:
Exosomes could help with longstanding medical issues from cancer diagnosis to sophisticated research tool. Now, they could potentially help with delivering potent antioxidants into the brain of Parkinson's patients.
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Researchers from the University of North Carolina at Chapel Hill show that exosomes loaded with catalase (shown in red) efficiently interact with neurons (shown in black) to protect them from the effects of Parkinson's disease.
Credit: Elena Batrakova/UNC Eshelman School of Pharmacy

Researchers at the University of North Carolina at Chapel Hill have used exosomes -- tiny bubbles of protein and fat produced naturally by cells -- to bypass the body's defenses and deliver a potent antioxidant directly to the brain to treat Parkinson's disease.

And what's the best way of getting her drug-packed exosomes to the brain? It looks like a simple nasal spray will do the trick, say Elena Batrakova and her colleagues at the UNC Eshelman School of Pharmacy's Center for Nanotechnology in Drug Delivery.

Batrakova and her colleagues extracted exosomes from immune cells and successfully loaded them with the enzyme catalase, a potent antioxidant that counters the neuron-killing inflammation responsible for Parkinson's and other degenerative neurological disorders. Their work was published in the Journal of Controlled Release.

This is the first time a large therapeutic protein like catalase has been delivered to the brain using exosomes. Getting drugs into the brain is extremely difficult in general because it is protected and isolated from the rest of the body by the blood-brain barrier, which is extremely selective about what is allowed to pass through.

Batrakova and her team at the pharmacy school harvested exosomes from macrophages, white blood cells that are responsible for clearing foreign material from the body. Exosomes are tiny spheres produced by cells to carry chemical messages. They are made of the same material that makes up cell membranes. Diseases like cancer and AIDS propagate throughout the body by hijacking exosomes.

"Exosomes are engineered by nature to be the perfect delivery vehicles for proteins and genetic material," Batrakova says. "Catalase is a huge protein, and it is almost impossible to deliver across the blood-brain barrier alone. We use exosomes from white blood cells, which are invisible to the immune system and easily interact and fuse with the blood-brain barrier to deliver their cargo across it."

Catalase counteracts the effects of free radicals, destructive molecules that are byproducts of cellular activity and especially prevalent in areas of chronic inflammation.

"Catalase is one of the most potent antioxidants in nature," Batrakova says. "One molecule of catalase can deactivate about one million free radicals per second, and it never stops because the enzyme is not consumed in the reaction. No small molecule drug even comes close to matching it in speed or efficiency."

Traditional drugs -- from cold medicine to chemotherapy -- are composed of small molecules of a few dozen atoms, typically. Biopharmaceuticals, or biologics, are proteins produced by living cells. Proteins such as catalase are tens of thousands of times larger than the small molecules that make up traditional drugs.

Batrakova's goal is to develop personalized treatments by loading proteins into exosomes that have been extracted from a patient's own white blood cells. These packages of medicine will be ignored by the patient's immune system, which works against unknown proteins as well as many synthetic delivery vehicles.


Story Source:

Materials provided by University of North Carolina at Chapel Hill. Note: Content may be edited for style and length.


Journal Reference:

  1. Matthew J. Haney, Natalia L. Klyachko, Yuling Zhao, Richa Gupta, Evgeniya G. Plotnikova, Zhijian He, Tejash Patel, Aleksandr Piroyan, Marina Sokolsky, Alexander V. Kabanov, Elena V. Batrakova. Exosomes as drug delivery vehicles for Parkinson's disease therapy. Journal of Controlled Release, 2015; 207: 18 DOI: 10.1016/j.jconrel.2015.03.033

Cite This Page:

University of North Carolina at Chapel Hill. "Cellular bubbles used to deliver Parkinson's meds directly to brain." ScienceDaily. ScienceDaily, 4 May 2015. <www.sciencedaily.com/releases/2015/05/150504141857.htm>.
University of North Carolina at Chapel Hill. (2015, May 4). Cellular bubbles used to deliver Parkinson's meds directly to brain. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2015/05/150504141857.htm
University of North Carolina at Chapel Hill. "Cellular bubbles used to deliver Parkinson's meds directly to brain." ScienceDaily. www.sciencedaily.com/releases/2015/05/150504141857.htm (accessed May 26, 2017).

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