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New analytical tool for fluorescence detection of double-stranded RNA

Date:
August 4, 2016
Source:
Tohoku University
Summary:
Ribonucleic acid binding fluorescent probes have been powerful and important analytical tools for the study of RNA structures and functions. Researchers report a new RNA probe that binds to double-stranded RNA (dsRNA) in a sequence-specific manner.
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Ribonucleic acid (RNA) binding fluorescent probes have been powerful and important analytical tools for the study of RNA structures and functions.

A research group led by Professor Seiichi Nishizawa at Tohoku University's Graduate School of Science has reported a new RNA probe that binds to double-stranded RNA (dsRNA) in a sequence-specific manner.

A fluorescent dye, thiazole orange (TO), is added to peptide nucleic acid (PNA). The probe exhibits a remarkable light-up response upon binding to the dsRNA by triplex formation.

The probe has a weak response to mismatch-containing dsRNA sequences, thus enabling sequence-selective fluorescence sensing of dsRNA at the single-base pair resolution. It also shows a preference for binding with dsRNA over dsDNA, which is an important selective process for future applications in a cellular environment where RNA and DNA co-exist.

In contrast to the conventional analytical method which is limited to single-stranded regions of RNA, the new analytical method allows for fluorescent sensing of target dsRNA structure and sequence for the first time.

It is expected that the probe will open up new possibilities for analyzing the functions of dsRNA-containing structures, which are closely related to various biological phenomena and diseases.


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Materials provided by Tohoku University. Note: Content may be edited for style and length.


Journal Reference:

  1. Takaya Sato, Yusuke Sato, Seiichi Nishizawa. Triplex-Forming Peptide Nucleic Acid Probe Having Thiazole Orange as a Base Surrogate for Fluorescence Sensing of Double-stranded RNA. Journal of the American Chemical Society, 2016; 138 (30): 9397 DOI: 10.1021/jacs.6b05554

Cite This Page:

Tohoku University. "New analytical tool for fluorescence detection of double-stranded RNA." ScienceDaily. ScienceDaily, 4 August 2016. <www.sciencedaily.com/releases/2016/08/160804101622.htm>.
Tohoku University. (2016, August 4). New analytical tool for fluorescence detection of double-stranded RNA. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2016/08/160804101622.htm
Tohoku University. "New analytical tool for fluorescence detection of double-stranded RNA." ScienceDaily. www.sciencedaily.com/releases/2016/08/160804101622.htm (accessed May 26, 2017).

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