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Highly sensitive test for SARS-CoV-2 may enable rapid point-of-care testing for COVID

Date:
June 17, 2021
Source:
Osaka University
Summary:
Researchers have created an intelligent nanopore system sensitive enough to detect single SARS-CoV-2 virus particles. By training a machine-learning algorithm, the platform was able to identify between various coronaviruses in just five minutes. This work may lead to fast and accurate point-of-care testing for COVID and other communicable diseases.
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A team of scientists headed by SANKEN (The Institute of Scientific and Industrial Research) at Osaka University demonstrated that single virus particles passing through a nanopore could be accurately identified using machine learning. The test platform they created was so sensitive that the coronaviruses responsible for the common cold, SARS, MERS, and COVID could be distinguished from each other. This work may lead to rapid, portable, and accurate screening tests for COVID and other viral diseases.

The global coronavirus pandemic has revealed the crucial need for rapid pathogen screening. However, the current gold-standard for detecting RNA viruses -- including SARS-CoV-2, the virus that causes COVID -- is reverse transcription-polymerase chain reaction (RT-PCR) testing. While accurate, this method is relatively slow, which hinders the timely interventions required to control an outbreak.

Now, scientists led by Osaka University have developed an intelligent nanopore system that can be used for the detection of SARS-CoV-2 virus particles. Using machine-learning methods, the platform can accurately discriminate between similarly sized coronaviruses responsible for different respiratory diseases. "Our innovative technology has high sensitivity and can even electrically identify single virus particles," first author Professor Masateru Taniguchi says. Using this platform, the researchers were able to achieve a sensitivity of 90% and a specificity of 96% for SARS-CoV-2 detection in just five minutes using clinical saliva samples.

To fabricate the device, nanopores just 300 nanometers in diameter were bored into a silicon nitride membrane. When a virus was pulled through a nanopore by the electrophoretic force, the opening became partially blocked. This temporarily decreased the ionic flow inside the nanopore, which was detected as a change in the electrical current. The current as a function of time provided information on the volume, structure, and surface charge of the target being analyzed. However, to interpret the subtle signals, which could be as small as a few nanoamps, machine learning was needed. The team used 40 PCR-positive and 40 PCR-negative saliva samples to train the algorithm.

"We expect that this research will enable rapid point-of-care and screening tests for SARS-CoV-2 without the need for RNA extraction," Professor Masateru Taniguchi explains. "A user-friendly and non-invasive method such as this is more amenable to immediate diagnosis in hospitals and screening in places where large crowds are gathered." The complete test platform consists of machine learning software on a server, a portable high-precision current measuring instrument, and cost-effective semiconducting nanopore modules. By using a machine-learning method, the researchers expect that this system can be adapted for use in the detection of emerging infectious diseases in the future. The team hopes that this approach will revolutionize public health and disease control.


Story Source:

Materials provided by Osaka University. Note: Content may be edited for style and length.


Journal Reference:

  1. Masateru Taniguchi, Shohei Minami, Chikako Ono, Rina Hamajima, Ayumi Morimura, Shigeto Hamaguchi, Yukihiro Akeda, Yuta Kanai, Takeshi Kobayashi, Wataru Kamitani, Yutaka Terada, Koichiro Suzuki, Nobuaki Hatori, Yoshiaki Yamagishi, Nobuei Washizu, Hiroyasu Takei, Osamu Sakamoto, Norihiko Naono, Kenji Tatematsu, Takashi Washio, Yoshiharu Matsuura, Kazunori Tomono. Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection. Nature Communications, 2021; 12 (1) DOI: 10.1038/s41467-021-24001-2

Cite This Page:

Osaka University. "Highly sensitive test for SARS-CoV-2 may enable rapid point-of-care testing for COVID." ScienceDaily. ScienceDaily, 17 June 2021. <www.sciencedaily.com/releases/2021/06/210617103612.htm>.
Osaka University. (2021, June 17). Highly sensitive test for SARS-CoV-2 may enable rapid point-of-care testing for COVID. ScienceDaily. Retrieved November 2, 2024 from www.sciencedaily.com/releases/2021/06/210617103612.htm
Osaka University. "Highly sensitive test for SARS-CoV-2 may enable rapid point-of-care testing for COVID." ScienceDaily. www.sciencedaily.com/releases/2021/06/210617103612.htm (accessed November 2, 2024).

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