Featured Research

from universities, journals, and other organizations

Scientists Develop Method For Comprehensive Proteome Analysis

Date:
April 15, 2009
Source:
Burnham Institute
Summary:
Investigators have deciphered a large percentage of the total protein complement (proteome) in Schizosaccharomyces pombe (S. pombe) fission yeast.

Investigators at Burnham Institute for Medical Research (Burnham) have deciphered a large percentage of the total protein complement (proteome) in Schizosaccharomyces pombe (S. pombe) fission yeast.

Related Articles


Laurence Brill, Khatereh Motamedchaboki, Ph.D. and lead investigator Dieter Wolf, Ph.D., developed the novel method, used to identify 4,600 proteins in the organism, using an array of sophisticated techniques. The research was published online on March 9 in the journal Methods.

“Analysis of the proteome of an organism tells us so much more than simple DNA sequence analysis,” said Dr. Wolf. “Proteome analysis gives us a snapshot of what proteins are being expressed in the cell at any given point in time. This can tell us how protein expression changes in response to certain stimuli and in disease states, which may help identify new biomarkers for diseases. We are now applying the methodology to protein profiling of human stem cells in collaboration with Burnham’s stem cell program director, Evan Snyder.”

The method developed by Burnham scientists involved digestion of the proteins into smaller peptides, then separation of the peptides based on electrostatic charge using strong anionic exchange chromatography. The peptides were further separated by molecular weight using high pressure liquid chromatography. Each of the individual peptides was detected and identified using mass spectrometry and database analysis. DNA analysis of the yeast’s genome predicts 5027 proteins. The team identified 4,600 proteins, which is not quite the entire proteome. The remaining 400 are only expressed during S. pombe’s mating state.

S. pombe is often used as a model organism to study DNA damage response and repair, cell division, stress responses and other aspects of cellular biology.


Story Source:

The above story is based on materials provided by Burnham Institute. Note: Materials may be edited for content and length.


Cite This Page:

Burnham Institute. "Scientists Develop Method For Comprehensive Proteome Analysis." ScienceDaily. ScienceDaily, 15 April 2009. <www.sciencedaily.com/releases/2009/04/090408145356.htm>.
Burnham Institute. (2009, April 15). Scientists Develop Method For Comprehensive Proteome Analysis. ScienceDaily. Retrieved April 1, 2015 from www.sciencedaily.com/releases/2009/04/090408145356.htm
Burnham Institute. "Scientists Develop Method For Comprehensive Proteome Analysis." ScienceDaily. www.sciencedaily.com/releases/2009/04/090408145356.htm (accessed April 1, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Wednesday, April 1, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Lion Makes Surprise Comeback in Gabon

Lion Makes Surprise Comeback in Gabon

AFP (Apr. 1, 2015) The noble lion has made a comeback in southeast Gabon, after disappearing for years, according to US wildlife organisation Panthera, which recently took live video footage of a male. Duration: 00:32 Video provided by AFP
Powered by NewsLook.com
Liberia Sees Resurgence of Drug Trafficking as Ebola Wanes

Liberia Sees Resurgence of Drug Trafficking as Ebola Wanes

AFP (Apr. 1, 2015) The governments of Liberia and Sierra Leone have been busy fighting the menace created by the deadly Ebola virus, but illicit drug lords have taken advantage of the situation to advance the drug trade. Duration: 01:12 Video provided by AFP
Powered by NewsLook.com
Gorilla Falls Into Zoo Moat

Gorilla Falls Into Zoo Moat

Reuters - Light News Video Online (Mar. 31, 2015) A gorilla comes to the rescue of her sister who fell into a moat in Israel&apos;s Safari zoo. Rough cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
California on Alert Over Surge in Sea Lion Strandings

California on Alert Over Surge in Sea Lion Strandings

AFP (Mar. 31, 2015) Since the start of the year, thousands of baby sea lions have washed up on beaches along the west coast of the United States. Marine animal care centers are working around the clock to save the stranded creatures. Duration: 02:06 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins