Featured Research

from universities, journals, and other organizations

Novel Mechanism Revealed For Increasing Recombinant Protein Yield In Tobacco

Date:
August 14, 2009
Source:
BioMed Central
Summary:
Elastin-like polypeptides cause plants to store GM proteins in special "protein bodies," insulating them from normal cellular degradation processes and increasing the overall protein yield. Researchers have visualized the mechanism by which the synthetic biopolymer increases the accumulation of recombinant proteins.

Elastin-like polypeptides (ELPs) cause plants to store GM proteins in special 'protein bodies', insulating them from normal cellular degradation processes and increasing the overall protein yield. Researchers have visualised the mechanism by which the synthetic biopolymer increases the accumulation of recombinant proteins.

Related Articles


Rima Menassa worked with a team of researchers from Agriculture and Agri-Food Canada in London, Ontario, to develop and test the ELP tags by targeting an ELP-green fluorescent protein (GFP) fusion to various organelles in the leaves of the tobacco plant. Tobacco is well-suited as a production system for recombinant proteins but the mechanism by which ELP fusions increase production yields in transgenic tobacco leaves was previously unknown. Menassa said, "ELP was shown to almost double the yield of GFP to 11% of total soluble protein when hyperexpressed in the endoplasmic reticulum (ER)".

Based on their confocal and electron microscopy analyses, the researchers suggest that ELP fusions targeted to the ER induce the formation of novel mobile protein body-like structures in leaves, which appear similar in size and morphology to the prolamin-based protein bodies naturally found in plant seeds. These bodies may be responsible for ELP's positive effect on recombinant protein accumulation by excluding the heterologous protein from normal physiological turnover.

The researchers targeted their ELP fusions to the cytoplasm, chloroplasts, apoplast and ER in Nicotiana benthamiana tobacco plants. They found that the ER was the only intracellular compartment in which the ELP significantly enhanced recombinant protein accumulation. They conclude, "An ER-targeted ELP fusion approach provides an effective strategy for depositing large amounts of concentrated heterologous protein within the limited space of the cell".


Story Source:

The above story is based on materials provided by BioMed Central. Note: Materials may be edited for content and length.


Journal Reference:

  1. Andrew J Conley, Jussi J Joensuu, Rima Menassa and Jim E Brandle. Induction of protein body formation in plant leaves by elastin-like polypeptide fusions. BMC Biology, (in press) [link]

Cite This Page:

BioMed Central. "Novel Mechanism Revealed For Increasing Recombinant Protein Yield In Tobacco." ScienceDaily. ScienceDaily, 14 August 2009. <www.sciencedaily.com/releases/2009/08/090806191936.htm>.
BioMed Central. (2009, August 14). Novel Mechanism Revealed For Increasing Recombinant Protein Yield In Tobacco. ScienceDaily. Retrieved October 25, 2014 from www.sciencedaily.com/releases/2009/08/090806191936.htm
BioMed Central. "Novel Mechanism Revealed For Increasing Recombinant Protein Yield In Tobacco." ScienceDaily. www.sciencedaily.com/releases/2009/08/090806191936.htm (accessed October 25, 2014).

Share This



More Plants & Animals News

Saturday, October 25, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Deep Sea 'mushroom' Could Be Early Branch on Tree of Life

Deep Sea 'mushroom' Could Be Early Branch on Tree of Life

Reuters - Innovations Video Online (Oct. 24, 2014) Miniature deep sea animals discovered off the Australian coast almost three decades ago are puzzling scientists, who say the organisms have proved impossible to categorise. Academics at the Natural History of Denmark have appealed to the world scientific community for help, saying that further information on Dendrogramma enigmatica and Dendrogramma discoides could answer key evolutionary questions. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Black Bear Cub Goes Sunday Shopping

Black Bear Cub Goes Sunday Shopping

Reuters - Light News Video Online (Oct. 23, 2014) Price check on honey? Bear cub startles Oregon drugstore shoppers. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com
Dances With Wolves in China's Wild West

Dances With Wolves in China's Wild West

AFP (Oct. 23, 2014) One man is on a mission to boost the population of wolves in China's violence-wracked far west. The animal - symbol of the Uighur minority there - is under threat with a massive human resettlement program in the region. Duration: 00:41 Video provided by AFP
Powered by NewsLook.com
Breakfast Debate: To Eat Or Not To Eat?

Breakfast Debate: To Eat Or Not To Eat?

Newsy (Oct. 23, 2014) Conflicting studies published in the same week re-ignited the debate over whether we should be eating breakfast. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins