A step toward understanding how hibernation protects hearts

The thirteen-lined ground squirrel hibernates all winter, but it regularly wakens about once a week. Each time the squirrels go back into slumber, they lower their body temperatures to nearly zero degrees Celsius and reduce their oxygen usage to 2 percent of the aroused state. But they do not develop ischemia, ventricular fibrillation and other dangerous circulatory problems that would occur in most mammals, including humans. These cycles mean their body temperature and metabolism fluctuate substantially, putting their hearts through the wringer over and over. Studying the molecular mechanisms behind how hibernators avoid this physiological roller coaster could one day help researchers understand the biology behind human heart failure, stroke and obesity. So, how do the squirrels survive such extremes? To answer this question, Matthew Andrews and his colleagues at the University of Minnesota used advanced proteogenomic techniques.

The researchers monitored changes in the ground squirrels' protein expression during different stages of hibernation. They compared these results against the squirrel's recently sequenced genome using a data analysis platform the team developed, called Galaxy-P. This proteogenomic analysis found dozens of proteins and protein signaling pathways that are expressed differently during hibernation. For example, the PPAR signaling pathway was upregulated during hibernation and highlights some of the proteins required to maintain heart function during that process. The team notes that these insights may be translated into therapies to prevent and treat cardiovascular disease in humans in the future.