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Architecture of Protein Complex Hints at Its Foundational Function in Chromosome Segregation

Date:
November 19, 2015
Source:
Whitehead Institute for Biomedical Research
Summary:
The organization of a protein complex that is critical during chromosome segregation has been revealed by researchers. Without the foundation it supplies, the link between chromosome and kinetochore would fail, as would chromosome segregation and cell division.
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Whitehead Institute researchers have revealed the architecture of a protein complex that plays a foundational role in the machine that directs chromosome segregation during cell division.

During chromosome segregation, the kinetochore serves as an attachment point for microtubules, which exert strong forces as they winch the chromosomes apart. In human cells, a protein complex termed the Constitutive Centromere-Associated Network (CCAN), is critical for recruiting the kinetochore to a specific point on each chromosome. Without the solid foundation provided by the 16-subunit CCAN, the link between chromosome and kinetochore would fail, as would chromosome segregation and cell division.

"The CCAN forms the critical connection between the DNA and the segregation machinery," says Whitehead Member Iain Cheeseman. "Learning about its organization can tell us how it targets specifically to the right part of the chromosome -- the centromere -- and the properties that underlie its role in kinetochore stability."

To study the CCAN organization, Kara McKinley, a graduate student in the Cheeseman lab, used the CRISPR-Cas9 genome editing system to conditionally knock out each subunit and also to introduce tags that cause the cell to chew up a given subunit upon addition of a drug. Previous research had relied on RNAi and other methods to knock down the proteins' expression, but none achieved the clean removal of the individual CCAN subcomplexes that is necessary for deciphering their position and function. In parallel, by studying all sixteen CCAN proteins biochemically, McKinley was able to refine the relationships seen in cells and establish how each individual subcomplex touches the others. The results of this work are published online this week in the journal Molecular Cell.

"The surprise was that each subcomplex needs to touch many other subcomplexes to be functional -- they're connected in a mesh, not a chain," says McKinley. "This property is crucial for building a stable structure for kinetochore assembly."

According to McKinley, this meshwork model helps explain other information about the CCAN. For example, the authors found that the connections between the subcomplexes help the CCAN tolerate the changes it is exposed to throughout the cell cycle. In addition, previous work in the field has indicated that these proteins are important in resisting the forces that pull on the kinetochore during chromosome segregation; the interconnected framework of interactions that the authors define is better able to withstand force than is a linear bridge.

The structure could also explain where and how kinetochores form. The histone protein CENP-A is the epigenetic mark for centromeres, but CENP-A nucleosomes can be sparsely integrated throughout the chromosome without recruiting a kinetochore. The authors hypothesize that the CCAN framework that they defined may span two or more CENP-A molecules when it creates a foundation for the kinetochore. Such crosslinking would only possible at high concentrations of CENP-A, as is true at the centromere.


Story Source:

Materials provided by Whitehead Institute for Biomedical Research. Original written by Nicole Giese Rura. Note: Content may be edited for style and length.


Journal Reference:

  1. Kara L. McKinley, Nikolina Sekulic, Lucie Y. Guo, Tonia Tsinman, Ben E. Black, Iain M. Cheeseman. The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface. Molecular Cell, 2015; DOI: 10.1016/j.molcel.2015.10.027

Cite This Page:

Whitehead Institute for Biomedical Research. "Architecture of Protein Complex Hints at Its Foundational Function in Chromosome Segregation." ScienceDaily. ScienceDaily, 19 November 2015. <www.sciencedaily.com/releases/2015/11/151119122621.htm>.
Whitehead Institute for Biomedical Research. (2015, November 19). Architecture of Protein Complex Hints at Its Foundational Function in Chromosome Segregation. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2015/11/151119122621.htm
Whitehead Institute for Biomedical Research. "Architecture of Protein Complex Hints at Its Foundational Function in Chromosome Segregation." ScienceDaily. www.sciencedaily.com/releases/2015/11/151119122621.htm (accessed May 23, 2017).

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