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A neuron's hardy bunch

Brain cell chatter persists even after dismantling key component of neurons' communication machinery

August 17, 2016
Harvard Medical School
The brain has exquisitely organized communication machinery that ensures ultrafast transmission of signals between neurons. Like cargo-carrying boats, tiny bubbles packed with neurotransmitters dock on standby, ready to unload their cargo upon demand. New research reveals that even when these 'docking stations' are absent or destroyed, a fleet of signaling vessels remain intact and ready to carry their signals across when called upon.

This is a normal mouse neuron with intact docking stations (in green). Docking stations, critical parts of a neuron's communication machinery, house neurotransmitter-packed bubbles (in red) that stand ready to launch when a trigger arrives. A new HMS study reveals that even when these docking stations are dismantled, neurons retain some of their ability to communicate with each other.
Credit: Harvard Medical School

Neuroscientists have long known that brain cells communicate with each other through the release of tiny bubbles packed with neurotransmitters -- a fleet of vessels docked along neuronal ends ready to launch when a trigger arrives.

Now a study conducted in mice by neurobiologists at Harvard Medical School reveals that dismantling the docking stations that house these signal-carrying vessels does not fully disrupt signal transmission between cells.

The team's experiments, described Aug. 17 in the journal Neuron, suggest the presence of mechanisms that help maintain partial communication despite serious structural aberrations.

"Our results not only address one of the most fundamental questions about neuronal activity and the way cells in the brain communicate with each other but uncover a few surprises too," said Pascal Kaeser, senior author on the study and assistant professor of neurobiology at HMS. "Our findings point to a fascinating underlying resilience in the nervous system."

Ultrafast signal transmission between neurons is vital for normal neurologic and cognitive function. In the brain, cell-to-cell communication occurs at the junction that connects two neurons -- a structure known as a synapse. At any given moment, neurotransmitter-carrying vesicles are on standby at designated docking stations, called active zones, each awaiting a trigger to release its load across the synaptic cleft and deliver it to the next neuron.

Signal strength and speed are determined by the number of vesicles ready and capable of releasing their cargo to the next neuron.

Neuroscientists have thus far surmised that destroying the docking stations that house neurotransmitter-loaded bubbles would cause all cell-to-cell communication to cease. The HMS team's findings suggest otherwise.

To examine the relationship between docking stations and signal transmission, researchers analyzed brain cells from mice genetically altered to lack two key building proteins, the absence of which led to the dismantling of the entire docking station.

When researchers measured signal strength in neurons with missing docking stations, they observed that those cells emitted much weaker signals when demand to transmit information was low. However, when stronger triggers were present, these cells transmitted remarkably robust signals, the researchers noticed.

"We would have guessed that signal transmission would cease altogether but it didn't," said Shan Shan Wang, a neuroscience graduate student in Kaeser's lab and a co-first author of the study. "Yet, neurons appear to retain some residual communication even with a key piece of their communication apparatus missing."

Elimination of one active zone building block, a protein called RIM, led to a three-quarter reduction in the pool of vesicles ready for release. Disruption of another key structural protein, ELKS, resulted in one-third fewer ready-to-deploy vesicles. When both proteins were missing, however, the total reduction in the number of releasable vesicles was far less than expected. More than 40 percent of a neuron's vesicles remained in a "ready to launch" state even with the entire docking station broken down and vesicles failing to dock.

The finding suggests that not all launch-ready vesicles need to be docked in the active zone when a trigger arrives. Neurons, the researchers say, appear to form a remote critical reserve of vesicles that can be quickly marshaled in times of high demand.

"In the absence of a docking sites, we observed that vesicles could be quickly recruited from afar when the need arises," said Richard Held, an HMS graduate student in neuroscience and co-first author on the paper.

The team cautions that any clinical implications remain far off, but say that their observations may help explain how defects in genes responsible for making neuronal docking stations may be implicated in a range of neurodevelopmental disorders.

Story Source:

Materials provided by Harvard Medical School. Original written by Ekaterina Pesheva. Note: Content may be edited for style and length.

Journal Reference:

  1. Shan Shan H. Wang, Richard G. Held, Man Yan Wong, Changliang Liu, Aziz Karakhanyan, Pascal S. Kaeser. Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking. Neuron, August 2016 DOI: 10.1016/j.neuron.2016.07.005

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Harvard Medical School. "A neuron's hardy bunch: Brain cell chatter persists even after dismantling key component of neurons' communication machinery." ScienceDaily. ScienceDaily, 17 August 2016. <>.
Harvard Medical School. (2016, August 17). A neuron's hardy bunch: Brain cell chatter persists even after dismantling key component of neurons' communication machinery. ScienceDaily. Retrieved May 23, 2017 from
Harvard Medical School. "A neuron's hardy bunch: Brain cell chatter persists even after dismantling key component of neurons' communication machinery." ScienceDaily. (accessed May 23, 2017).